SPR Kit ELISA

N° du produit ABIN5658710

Détail du produit SPR Kit ELISA

Antigène: SPR (Sepiapterin Reductase (SPR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Sepiapterin Reductase (SPR). No significant cross-reactivity or interference between Sepiapterin Reductase (SPR) and analogues was observed.

Sensibilité: 0.108 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sepiapterin Reductase (SPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sepiapterin Reductase (SPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sepiapterin Reductase (SPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sepiapterin Reductase (SPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sepiapterin Reductase (SPR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sepiapterin Reductase (SPR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SPR (Sepiapterin Reductase (SPR))

Autre désignation: Sepiapterin Reductase (SPR Produits)

Synonymes: SDR38C1 Kit ELISA, AA409688 Kit ELISA, ENSMUSG00000071345 Kit ELISA, Gm10328 Kit ELISA, CG12117 Kit ELISA, Dmel\\CG12117 Kit ELISA, SR Kit ELISA, sptr Kit ELISA, xspr Kit ELISA, DDBDRAFT_0188686 Kit ELISA, DDBDRAFT_0191275 Kit ELISA, DDB_0188686 Kit ELISA, DDB_0191275 Kit ELISA, zgc:194446 Kit ELISA, sepiapterin reductase Kit ELISA, Sepiapterin reductase Kit ELISA, sepiapterin reductase L homeolog Kit ELISA, sepiapterin reductase b Kit ELISA, SPR Kit ELISA, Spr Kit ELISA, Sptr Kit ELISA, spr.L Kit ELISA, sprE Kit ELISA, CpipJ_CPIJ000511 Kit ELISA, sr Kit ELISA, spr Kit ELISA, sprb Kit ELISA

Sujet:

Gene Name: Sepiapterin Reductase

Gene Aliases: SDR38C1, 7,8-Dihydrobiopterin:NADP+ Oxidoreductase, Short Chain Dehydrogenase/Reductase Family 38C,Member 1

ID gène: 6697

UniProt: P35270

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration