SPTLC2 Kit ELISA

N° du produit ABIN5658720

Détail du produit SPTLC2 Kit ELISA

Antigène: SPTLC2 (serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2). No significant cross-reactivity or interference between Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SPTLC2 (serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2))

Autre désignation: Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2 Produits)

Synonymes: HSN1C Kit ELISA, LCB2 Kit ELISA, LCB2A Kit ELISA, NSAN1C Kit ELISA, SPT2 Kit ELISA, hLCB2a Kit ELISA, sptlc2 Kit ELISA, zgc:110661 Kit ELISA, AI173915 Kit ELISA, mKIAA0526 Kit ELISA, Pomt2 Kit ELISA, LCB2a Kit ELISA, SPTLC2 Kit ELISA, serine palmitoyltransferase long chain base subunit 2 Kit ELISA, serine palmitoyltransferase 2 Kit ELISA, serine palmitoyltransferase, long chain base subunit 2 S homeolog Kit ELISA, serine palmitoyltransferase, long chain base subunit 2a Kit ELISA, serine palmitoyltransferase, long chain base subunit 2 Kit ELISA, SPTLC2 Kit ELISA, LOC100446763 Kit ELISA, sptlc2 Kit ELISA, sptlc2.S Kit ELISA, sptlc2a Kit ELISA, Sptlc2 Kit ELISA

Sujet:

Gene Name: Serine Palmitoyltransferase, Long Chain Base Subunit 2

Gene Aliases: LCB2, SPT2, Long chain base biosynthesis protein 2, Serine-palmitoyl-CoA transferase 2

ID gène: 20773

UniProt: P97363