LKB1 Kit ELISA

N° du produit ABIN5658730

Détail du produit LKB1 Kit ELISA

Antigène: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Serine/Threonine Kinase 11 (STK11). No significant cross-reactivity or interference between Serine/Threonine Kinase 11 (STK11) and analogues was observed.

Sensibilité: 0.051 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/Threonine Kinase 11 (STK11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine/Threonine Kinase 11 (STK11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine/Threonine Kinase 11 (STK11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine/Threonine Kinase 11 (STK11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine/Threonine Kinase 11 (STK11) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine/Threonine Kinase 11 (STK11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Autre désignation: Serine/Threonine Kinase 11 (STK11 Produits)

Synonymes: pjs Kit ELISA, LKB1 Kit ELISA, XEEK1 Kit ELISA, Stk11 Kit ELISA, PJS Kit ELISA, hLKB1 Kit ELISA, AA408040 Kit ELISA, Lkb1 Kit ELISA, Par-4 Kit ELISA, R75140 Kit ELISA, mLKB1 Kit ELISA, wu:fj61a05 Kit ELISA, zgc:110180 Kit ELISA, xeek1 Kit ELISA, serine/threonine kinase 11 Kit ELISA, polarization-related protein LKB1 Kit ELISA, serine/threonine kinase 11 L homeolog Kit ELISA, STK11 Kit ELISA, stk11 Kit ELISA, LOC662493 Kit ELISA, Stk11 Kit ELISA, stk11.L Kit ELISA

Sujet:

Gene Name: Serine/Threonine Kinase 11

Gene Aliases: LKB1, PJS, Polarization-Related Protein LKB1, Peutz-Jeghers Syndrome, Liver kinase B1, Renal carcinoma antigen NY-REN-19

ID gène: 20869

UniProt: Q9WTK7

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg