SIRPA Kit ELISA

N° du produit ABIN5658790

Détail du produit SIRPA Kit ELISA

Antigène: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Signal Regulatory Protein Alpha (SIRPa). No significant cross-reactivity or interference between Signal Regulatory Protein Alpha (SIRPa) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Regulatory Protein Alpha (SIRPa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Regulatory Protein Alpha (SIRPa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Regulatory Protein Alpha (SIRPa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Regulatory Protein Alpha (SIRPa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Regulatory Protein Alpha (SIRPa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Regulatory Protein Alpha (SIRPa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Autre désignation: Signal Regulatory Protein Alpha (SIRPA Produits)

Synonymes: BIT Kit ELISA, CD172A Kit ELISA, MFR Kit ELISA, MYD-1 Kit ELISA, P84 Kit ELISA, PTPNS1 Kit ELISA, SHPS1 Kit ELISA, SIRP Kit ELISA, SIRPA Kit ELISA, SIRP-A1 Kit ELISA, SIRP-B1 Kit ELISA, AI835480 Kit ELISA, Bit Kit ELISA, CD172a Kit ELISA, Ptpns1 Kit ELISA, SHP-1 Kit ELISA, SHPS-1 Kit ELISA, swc3 Kit ELISA, signal regulatory protein alpha Kit ELISA, myeloperoxidase Kit ELISA, signal-regulatory protein alpha Kit ELISA, tyrosine-protein phosphatase non-receptor type substrate 1 Kit ELISA, SIRPA Kit ELISA, MPO Kit ELISA, LOC458039 Kit ELISA, LOC100400378 Kit ELISA, LOC100590224 Kit ELISA, Sirpa Kit ELISA

Sujet:

Gene Name: Signal Regulatory Protein Alpha

Gene Aliases: CD172a, SIRP-A, P84, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1, Macrophage fusion receptor

ID gène: 140885

UniProt: P78324