SUMO1 Kit ELISA

N° du produit ABIN5658838

Détail du produit SUMO1 Kit ELISA

Antigène: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Small Ubiquitin Related Modifier Protein 1 (SUMO1). No significant cross-reactivity or interference between Small Ubiquitin Related Modifier Protein 1 (SUMO1) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Ubiquitin Related Modifier Protein 1 (SUMO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Small Ubiquitin Related Modifier Protein 1 (SUMO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Ubiquitin Related Modifier Protein 1 (SUMO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Small Ubiquitin Related Modifier Protein 1 (SUMO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Small Ubiquitin Related Modifier Protein 1 (SUMO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Small Ubiquitin Related Modifier Protein 1 (SUMO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Autre désignation: Small Ubiquitin Related Modifier Protein 1 (SUMO1 Produits)

Synonymes: DAP1 Kit ELISA, GMP1 Kit ELISA, OFC10 Kit ELISA, PIC1 Kit ELISA, SENP2 Kit ELISA, SMT3 Kit ELISA, SMT3C Kit ELISA, SMT3H3 Kit ELISA, UBL1 Kit ELISA, hm:zeh0670 Kit ELISA, wu:fb74c02 Kit ELISA, wu:fd12c02 Kit ELISA, zgc:65934 Kit ELISA, zgc:85634 Kit ELISA, SUMO1 Kit ELISA, dap1 Kit ELISA, gmp1 Kit ELISA, ofc10 Kit ELISA, pic1 Kit ELISA, senp2 Kit ELISA, smt3 Kit ELISA, smt3c Kit ELISA, sumo-1 Kit ELISA, sumo1 Kit ELISA, ubl1 Kit ELISA, xsumo-1 Kit ELISA, xsumo1 Kit ELISA, ARABIDOPSIS THALIANA SMALL UBIQUITIN-LIKE MODIFIER 1 Kit ELISA, ATSUMO1 Kit ELISA, F10M23.180 Kit ELISA, F10M23_180 Kit ELISA, SMALL UBIQUITIN-LIKE MODIFIER 1 Kit ELISA, SUM1 Kit ELISA, SUMO 1 Kit ELISA, small ubiquitin-like modifier 1 Kit ELISA, pigSUMO1 Kit ELISA, SENTRIN Kit ELISA, SMTP3 Kit ELISA, SUMO-1 Kit ELISA, Smt3C Kit ELISA, Ubl1 Kit ELISA, small ubiquitin-like modifier 1 Kit ELISA, small ubiquitin-like modifier 1 S homeolog Kit ELISA, small ubiquitin-related protein 1 Kit ELISA, SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) Kit ELISA, SMT3 suppressor of mif two 3 homolog 1 (yeast) Kit ELISA, small ubiquitin-like modifier 1 L homeolog Kit ELISA, Sumo1 Kit ELISA, SUMO1 Kit ELISA, sumo1 Kit ELISA, sumo1.S Kit ELISA, sumo-1 Kit ELISA, sumo1.L Kit ELISA

Sujet:

Gene Name: Small Ubiquitin Related Modifier Protein 1

Gene Aliases: GMP1, OFC10, PIC1, SMT3, SMT3C, SMT3H3, SUMO-1, UBL1, GAP-modifying protein 1, Ubiquitin-homology domain protein PIC1

ID gène: 7341

UniProt: P63165

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway