SUMO2 Kit ELISA

N° du produit ABIN5658841

Détail du produit SUMO2 Kit ELISA

Antigène: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Small Ubiquitin Related Modifier Protein 2 (SUMO2). No significant cross-reactivity or interference between Small Ubiquitin Related Modifier Protein 2 (SUMO2) and analogues was observed.

Sensibilité: 0.126 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Ubiquitin Related Modifier Protein 2 (SUMO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Small Ubiquitin Related Modifier Protein 2 (SUMO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Ubiquitin Related Modifier Protein 2 (SUMO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Small Ubiquitin Related Modifier Protein 2 (SUMO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Small Ubiquitin Related Modifier Protein 2 (SUMO2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Small Ubiquitin Related Modifier Protein 2 (SUMO2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Autre désignation: Small Ubiquitin Related Modifier Protein 2 (SUMO2 Produits)

Synonymes: HSMT3 Kit ELISA, SMT3B Kit ELISA, SMT3H2 Kit ELISA, SUMO3 Kit ELISA, Smt3A Kit ELISA, SMT3A Kit ELISA, SMT3H1 Kit ELISA, SUMO-3 Kit ELISA, Smt3B Kit ELISA, Smt3h2 Kit ELISA, sumo2 Kit ELISA, zgc:92241 Kit ELISA, SUMO2 Kit ELISA, SUMO-2 Kit ELISA, Smt3b Kit ELISA, hsmt3 Kit ELISA, smt3b Kit ELISA, smt3h2 Kit ELISA, sumo-2 Kit ELISA, PSMT3 Kit ELISA, ATSUMO2 Kit ELISA, MCO15.11 Kit ELISA, MCO15_11 Kit ELISA, SMALL UBIQUITIN-LIKE MODIFIER 2 Kit ELISA, SUM2 Kit ELISA, SUMO 2 Kit ELISA, small ubiquitin-like modifier 2 Kit ELISA, small ubiquitin-like modifier 2 Kit ELISA, small ubiquitin-like modifier 3 Kit ELISA, small ubiquitin-like modifier 2b Kit ELISA, small ubiquitin-related modifier 2 Kit ELISA, small ubiquitin-like modifier 2 S homeolog Kit ELISA, small ubiquitin-like modifier 2 L homeolog Kit ELISA, SUMO2 Kit ELISA, SUMO3 Kit ELISA, Sumo2 Kit ELISA, sumo2b Kit ELISA, LOC714892 Kit ELISA, sumo2.S Kit ELISA, sumo2.L Kit ELISA

Sujet:

Gene Name: Small Ubiquitin Related Modifier Protein 2

Gene Aliases: HSMT3, SMT3B, SMT3H2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B

ID gène: 6613

UniProt: P61956

Pathways: Methionine Biosynthetic Process