SNAIL Kit ELISA

N° du produit ABIN5658844

Détail du produit SNAIL Kit ELISA

Antigène: SNAIL (SNAI1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Snail Homolog 1 (SNAI1). No significant cross-reactivity or interference between Snail Homolog 1 (SNAI1) and analogues was observed.

Sensibilité: 0.063 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Snail Homolog 1 (SNAI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Snail Homolog 1 (SNAI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Snail Homolog 1 (SNAI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Snail Homolog 1 (SNAI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Snail Homolog 1 (SNAI1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Snail Homolog 1 (SNAI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SNAIL (SNAI1)

Autre désignation: Snail Homolog 1 (SNAI1 Produits)

Synonymes: SLUGH2 Kit ELISA, SNA Kit ELISA, SNAH Kit ELISA, SNAIL Kit ELISA, SNAIL1 Kit ELISA, dJ710H13.1 Kit ELISA, AI194338 Kit ELISA, Sna Kit ELISA, Sna1 Kit ELISA, Snail Kit ELISA, Snail1 Kit ELISA, SNAI1 Kit ELISA, BG:DS01845.1 Kit ELISA, CG3956 Kit ELISA, Dmel\\CG3956 Kit ELISA, Sco Kit ELISA, br28 Kit ELISA, br29 Kit ELISA, l(2)35Db Kit ELISA, l(2)br28 Kit ELISA, l(2)br29 Kit ELISA, l(3)br28 Kit ELISA, l35Db Kit ELISA, sn Kit ELISA, Xsnail Kit ELISA, sna Kit ELISA, snail1 Kit ELISA, xSna Kit ELISA, Snai Kit ELISA, sna2 Kit ELISA, snail2 Kit ELISA, cb643 Kit ELISA, sna-1 Kit ELISA, sna1 Kit ELISA, snai1 Kit ELISA, snail1a Kit ELISA, zgc:109820 Kit ELISA, snail family transcriptional repressor 1 Kit ELISA, snail family zinc finger 1 Kit ELISA, snail homolog 1 (Drosophila) Kit ELISA, snail Kit ELISA, zinc-finger transcription factor Snail Kit ELISA, snail family zinc finger 1 S homeolog Kit ELISA, protein escargot Kit ELISA, transcription factor protein Kit ELISA, snail zinc finger protein Kit ELISA, snail family zinc finger 1b Kit ELISA, snail family zinc finger 1a Kit ELISA, SNAI1 Kit ELISA, Snai1 Kit ELISA, sna Kit ELISA, snail Kit ELISA, snai1.S Kit ELISA, LOC410464 Kit ELISA, snai1b Kit ELISA, snai1a Kit ELISA

Sujet:

Gene Name: Snail Homolog 1

Gene Aliases: SLUGH2, SNA, SNAH, Snail 1 Zinc Finger Protein

ID gène: 6615

UniProt: O95863

Pathways: Negative Regulation of intrinsic apoptotic Signaling