SLC3A2 Kit ELISA

N° du produit ABIN5658861

Détail du produit SLC3A2 Kit ELISA

Antigène: SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 3, Member 2 (SLC3A2). No significant cross-reactivity or interference between Solute Carrier Family 3, Member 2 (SLC3A2) and analogues was observed.

Sensibilité: 0.061 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 3, Member 2 (SLC3A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 3, Member 2 (SLC3A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 3, Member 2 (SLC3A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 3, Member 2 (SLC3A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 3, Member 2 (SLC3A2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 3, Member 2 (SLC3A2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SLC3A2 (Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2))

Autre désignation: Solute Carrier Family 3, Member 2 (SLC3A2 Produits)

Synonymes: 4F2 Kit ELISA, 4F2HC Kit ELISA, 4T2HC Kit ELISA, CD98 Kit ELISA, CD98HC Kit ELISA, MDU1 Kit ELISA, NACAE Kit ELISA, Mdu1 Kit ELISA, MGC53951 Kit ELISA, slc3a2 Kit ELISA, MGC53965 Kit ELISA, SLC3A2 Kit ELISA, AI314110 Kit ELISA, Cd98 Kit ELISA, Ly-10 Kit ELISA, Ly-m10 Kit ELISA, Ly10 Kit ELISA, Mgp-2hc Kit ELISA, fc65h12 Kit ELISA, wu:fc65h12 Kit ELISA, id:ibd1327 Kit ELISA, sb:cb519 Kit ELISA, wu:fe14a07 Kit ELISA, wu:fq18d12 Kit ELISA, zgc:55813 Kit ELISA, solute carrier family 3 member 2 Kit ELISA, solute carrier family 3 (amino acid transporter heavy chain), member 2 L homeolog Kit ELISA, solute carrier family 3 (amino acid transporter heavy chain), member 2 S homeolog Kit ELISA, solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 Kit ELISA, solute carrier family 3 (amino acid transporter heavy chain), member 2a Kit ELISA, solute carrier family 3 (amino acid transporter heavy chain), member 2b Kit ELISA, SLC3A2 Kit ELISA, Slc3a2 Kit ELISA, slc3a2.L Kit ELISA, slc3a2.S Kit ELISA, slc3a2a Kit ELISA, slc3a2b Kit ELISA

Sujet:

Gene Name: Solute Carrier Family 3, Member 2

Gene Aliases: SLC3A2, CD98HC, 4F2, 4F2HC, 4T2HC, MDU1, NACAE, Activators Of Dibasic And Neutral Amino Acid Transport, Antigen Identified By Monoclonal Antibodies 4F2, CD98 Heavy Chain

ID gène: 6520

UniProt: P08195