SORD Kit ELISA

N° du produit ABIN5658893

Détail du produit SORD Kit ELISA

Antigène: SORD (Sorbitol Dehydrogenase (SORD))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Sorbitol Dehydrogenase (SDH). No significant cross-reactivity or interference between Sorbitol Dehydrogenase (SDH) and analogues was observed.

Sensibilité: 0.66 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorbitol Dehydrogenase (SDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorbitol Dehydrogenase (SDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorbitol Dehydrogenase (SDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sorbitol Dehydrogenase (SDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SORD (Sorbitol Dehydrogenase (SORD))

Autre désignation: Sorbitol Dehydrogenase (SORD Produits)

Synonymes: SORD1 Kit ELISA, Sdh-1 Kit ELISA, Sdh1 Kit ELISA, Sodh-1 Kit ELISA, SDH Kit ELISA, sord Kit ELISA, MGC83437 Kit ELISA, BmSDH Kit ELISA, PSPTO0363 Kit ELISA, SODH-1 Kit ELISA, RATSDHE1 Kit ELISA, SDH2 Kit ELISA, Sdh Kit ELISA, Sdhe1 Kit ELISA, TDH Kit ELISA, 4733401P19Rik Kit ELISA, AI836084 Kit ELISA, CGI-97 Kit ELISA, SDS Kit ELISA, zgc:63674 Kit ELISA, sorbitol dehydrogenase Kit ELISA, sorbitol dehydrogenase L homeolog Kit ELISA, Sorbitol dehydrogenase Kit ELISA, serine dehydratase Kit ELISA, SBDS ribosome maturation factor Kit ELISA, SORD Kit ELISA, Sord Kit ELISA, sord.L Kit ELISA, sord Kit ELISA, Sdh Kit ELISA, LOC733025 Kit ELISA, polS Kit ELISA, RS_RS10765 Kit ELISA, sdh Kit ELISA, smoS Kit ELISA, Rsph17029_1731 Kit ELISA, Srd Kit ELISA, RSKD131_1411 Kit ELISA, SDS Kit ELISA, Sds Kit ELISA, Sbds Kit ELISA

Sujet:

Gene Name: Sorbitol Dehydrogenase

Gene Aliases: SORD, L-iditol 2-dehydrogenase

ID gène: 24788

UniProt: P27867