SRI Kit ELISA

N° du produit ABIN5658894

Détail du produit SRI Kit ELISA

Antigène: SRI (Sorcin (SRI))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Sorcin (SRI). No significant cross-reactivity or interference between Sorcin (SRI) and analogues was observed.

Sensibilité: 0.119 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorcin (SRI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorcin (SRI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorcin (SRI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sorcin (SRI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sorcin (SRI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sorcin (SRI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SRI (Sorcin (SRI))

Autre désignation: Sorcin (SRI Produits)

Synonymes: zgc:64070 Kit ELISA, sri Kit ELISA, SRI Kit ELISA, CP-22 Kit ELISA, CP22 Kit ELISA, SCN Kit ELISA, V19 Kit ELISA, 2210417O06Rik Kit ELISA, 2900070H08Rik Kit ELISA, Sor Kit ELISA, sorcin Kit ELISA, Sorcin Kit ELISA, sorcin L homeolog Kit ELISA, sorcin S homeolog Kit ELISA, SRI Kit ELISA, sri Kit ELISA, EDI_060410 Kit ELISA, EDI_160670 Kit ELISA, EDI_002860 Kit ELISA, EDI_076560 Kit ELISA, CpipJ_CPIJ004933 Kit ELISA, CpipJ_CPIJ008581 Kit ELISA, Tsp_05707 Kit ELISA, sri.L Kit ELISA, sri.S Kit ELISA, Sri Kit ELISA

Sujet:

Gene Name: Sorcin

Gene Aliases: SCN, CP-22, V19, 22 kDa protein

ID gène: 6717

UniProt: P30626

Pathways: Transition Metal Ion Homeostasis, Maintenance of Protein Location, Negative Regulation of Transporter Activity