SNX9 Kit ELISA

N° du produit ABIN5658899

Détail du produit SNX9 Kit ELISA

Antigène: SNX9 (Sorting Nexin 9 (SNX9))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Sorting Nexin 9 (SNX9). No significant cross-reactivity or interference between Sorting Nexin 9 (SNX9) and analogues was observed.

Sensibilité: 0.052 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorting Nexin 9 (SNX9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorting Nexin 9 (SNX9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorting Nexin 9 (SNX9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sorting Nexin 9 (SNX9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sorting Nexin 9 (SNX9) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sorting Nexin 9 (SNX9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SNX9 (Sorting Nexin 9 (SNX9))

Autre désignation: Sorting Nexin 9 (SNX9 Produits)

Synonymes: si:bz36d5.4 Kit ELISA, snx9 Kit ELISA, zgc:113455 Kit ELISA, MGC78823 Kit ELISA, SNX9 Kit ELISA, SDP1 Kit ELISA, SH3PX1 Kit ELISA, SH3PXD3A Kit ELISA, WISP Kit ELISA, 2700073N08Rik Kit ELISA, sorting nexin 9a Kit ELISA, sorting nexin 9 Kit ELISA, sorting nexin 9 L homeolog Kit ELISA, snx9a Kit ELISA, SNX9 Kit ELISA, snx9.L Kit ELISA, snx9 Kit ELISA, Snx9 Kit ELISA

Sujet:

Gene Name: Sorting Nexin 9

Gene Aliases: SDP1, SH3PX1, SH3PXD3A, WISP, SH3 and PX domain-containing protein 1, SH3 and PX domain-containing protein 3A

ID gène: 51429

UniProt: Q9Y5X1