SCD Kit ELISA

N° du produit ABIN5658943

Détail du produit SCD Kit ELISA

Antigène: SCD (Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Stearoyl Coenzyme A Desaturase (SCD). No significant cross-reactivity or interference between Stearoyl Coenzyme A Desaturase (SCD) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stearoyl Coenzyme A Desaturase (SCD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stearoyl Coenzyme A Desaturase (SCD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stearoyl Coenzyme A Desaturase (SCD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stearoyl Coenzyme A Desaturase (SCD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stearoyl Coenzyme A Desaturase (SCD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stearoyl Coenzyme A Desaturase (SCD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SCD (Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD))

Autre désignation: Stearoyl Coenzyme A Desaturase (SCD Produits)

Synonymes: FADS5 Kit ELISA, MSTP008 Kit ELISA, SCD1 Kit ELISA, SCDOS Kit ELISA, AA589638 Kit ELISA, AI265570 Kit ELISA, Scd Kit ELISA, Scd-1 Kit ELISA, ab Kit ELISA, SCD Kit ELISA, fads5 Kit ELISA, scd1 Kit ELISA, scdos Kit ELISA, F27J15.17 Kit ELISA, F27J15_17 Kit ELISA, STOMATAL CYTOKINESIS-DEFECTIVE 1 Kit ELISA, cds2 Kit ELISA, desaturase Kit ELISA, stearoyl-CoA desaturase Kit ELISA, acyl-CoA desaturase Kit ELISA, Acyl-CoA desaturase Kit ELISA, stearoyl-Coenzyme A desaturase 1 Kit ELISA, stearoyl-CoA desaturase (delta-9-desaturase) Kit ELISA, stearoyl-CoA desaturase (delta-9-desaturase) S homeolog Kit ELISA, stomatal cytokinesis defective / SCD1 protein (SCD1) Kit ELISA, acyl-CoA desaturase-like Kit ELISA, SCD Kit ELISA, CIMG_08158 Kit ELISA, VIBHAR_RS23280 Kit ELISA, acod Kit ELISA, Scd1 Kit ELISA, Scd Kit ELISA, scd Kit ELISA, LOC100346561 Kit ELISA, scd.S Kit ELISA, SCD1 Kit ELISA, LOC100719661 Kit ELISA, LOC101835884 Kit ELISA, LOC109101093 Kit ELISA

Sujet:

Gene Name: Stearoyl Coenzyme A Desaturase

Gene Aliases: FADS5, SCDOS, Fatty Acid desaturase 5, Delta-9-Desaturase, Stearoyl-CoA Desaturase Opposite Strand

Pathways: Brown Fat Cell Differentiation