STS Kit ELISA

N° du produit ABIN5658962

Détail du produit STS Kit ELISA

Antigène: STS (Steroid Sulfatase (Microsomal), Isozyme S (STS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Steroid Sulfatase Isozyme S (STS). No significant cross-reactivity or interference between Steroid Sulfatase Isozyme S (STS) and analogues was observed.

Sensibilité: 0.29 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroid Sulfatase Isozyme S (STS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Steroid Sulfatase Isozyme S (STS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Steroid Sulfatase Isozyme S (STS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Steroid Sulfatase Isozyme S (STS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Steroid Sulfatase Isozyme S (STS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Steroid Sulfatase Isozyme S (STS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: STS (Steroid Sulfatase (Microsomal), Isozyme S (STS))

Autre désignation: Steroid Sulfatase Isozyme S (STS Produits)

Synonymes: ARSC Kit ELISA, ARSC1 Kit ELISA, ASC Kit ELISA, ES Kit ELISA, SSDD Kit ELISA, XLI Kit ELISA, abcg2 Kit ELISA, ArsC Kit ELISA, Sts Kit ELISA, si:ch211-271l9.1 Kit ELISA, steroid sulfatase Kit ELISA, breast cancer resistance protein Kit ELISA, Steryl-sulfatase Kit ELISA, steryl-sulfatase Kit ELISA, steroid sulfatase (microsomal), isozyme S Kit ELISA, STS Kit ELISA, abcg2 Kit ELISA, Sts Kit ELISA, Plav_0360 Kit ELISA, Psta_3963 Kit ELISA, Runsl_5106 Kit ELISA, LOC100712701 Kit ELISA, sts Kit ELISA

Sujet:

Gene Name: Steroid Sulfatase Isozyme S

Gene Aliases: ARSC, ARSC1, ASC, ES, SSDD, Arylsulfatase C, Steryl-sulfatase, Steryl-sulfate sulfohydrolase

ID gène: 412

UniProt: P08842

Pathways: Steroid Hormone Biosynthesis