SOD1 Kit ELISA
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- Antigène Voir toutes SOD1 Kits ELISA
- SOD1 (Superoxide Dismutase 1, Soluble (SOD1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 62.5 pg/mL - 4000 pg/mL
- Seuil minimal de détection
- 62.5 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase 1, Soluble (SOD1). No significant cross-reactivity or interference between Superoxide Dismutase 1, Soluble (SOD1) and analogues was observed.
- Sensibilité
- 26.7 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase 1, Soluble (SOD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutase 1, Soluble (SOD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase 1, Soluble (SOD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superoxide Dismutase 1, Soluble (SOD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase 1, Soluble (SOD1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase 1, Soluble (SOD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes SOD1 Kits ELISA
- SOD1 (Superoxide Dismutase 1, Soluble (SOD1))
- Autre désignation
- Superoxide Dismutase 1, Soluble (SOD1 Produits)
- Synonymes
- LOC692639 Kit ELISA, ALS Kit ELISA, ALS1 Kit ELISA, IPOA Kit ELISA, SOD Kit ELISA, hSod1 Kit ELISA, homodimer Kit ELISA, CG11793 Kit ELISA, Cu Kit ELISA, Cu-Zn SOD Kit ELISA, Cu/Zn SOD Kit ELISA, Cu/Zn sod Kit ELISA, Cu/Zn superoxide dismutase Kit ELISA, Cu/ZnSOD Kit ELISA, CuSOD Kit ELISA, CuZn SOD Kit ELISA, CuZn-SOD Kit ELISA, CuZn-SOD1 Kit ELISA, CuZnSOD Kit ELISA, Cu[2+]/Zn[2+]SOD Kit ELISA, Dmel\\CG11793 Kit ELISA, G Kit ELISA, Mn SOD Kit ELISA, SOD-1 Kit ELISA, SOD1 Kit ELISA, Sod-1 Kit ELISA, Sod1 Kit ELISA, To Kit ELISA, To-1 Kit ELISA, Zn SOD Kit ELISA, Zn Sod Kit ELISA, Zn-SOD Kit ELISA, ZnSod Kit ELISA, cSOD Kit ELISA, cSod Kit ELISA, dSOD1 Kit ELISA, l(3)108 Kit ELISA, l(3)68Af' Kit ELISA, l(3)G Kit ELISA, sod Kit ELISA, sod1 Kit ELISA, CU/ZN-SOD Kit ELISA, SODC Kit ELISA, DKFZP469M1833 Kit ELISA, B430204E11Rik Kit ELISA, Cu/Zn-SOD Kit ELISA, Ipo-1 Kit ELISA, Ipo1 Kit ELISA, SOD1L1 Kit ELISA, XSODB Kit ELISA, als Kit ELISA, als1 Kit ELISA, ipoa Kit ELISA, sod1-a Kit ELISA, ZSOD Kit ELISA, cuzn Kit ELISA, Cu/Zn superoxide dismutase Kit ELISA, superoxide dismutase 1 Kit ELISA, Superoxide dismutase 1 Kit ELISA, superoxide dismutase 1, soluble Kit ELISA, superoxide dismutase 1 S homeolog Kit ELISA, Superoxide dismutase [Cu-Zn] Kit ELISA, superoxide dismutase 1 L homeolog Kit ELISA, superoxide dismutase [Cu-Zn]-like Kit ELISA, superoxide dismutase [Cu-Zn] Kit ELISA, superoxide dismutase Sod1 Kit ELISA, SOD Kit ELISA, SOD1 Kit ELISA, Sod1 Kit ELISA, sod1 Kit ELISA, sod1.S Kit ELISA, sod-1 Kit ELISA, sod1.L Kit ELISA, LOC101451855 Kit ELISA, LOC101115136 Kit ELISA
- Sujet
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Gene Name: Superoxide Dismutase 1, Soluble
Gene Aliases: ALS, ALS1, IPOA, Amyotrophic Lateral Sclerosis 1,Adult, Superoxide dismutase [Cu-Zn]
- ID gène
- 6647
- UniProt
- P00441
- Pathways
- Sensory Perception of Sound, Transition Metal Ion Homeostasis
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