SOD2 Kit ELISA
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- Antigène Voir toutes SOD2 Kits ELISA
- SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))
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Reactivité
- Rat
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 31.25 pg/mL - 2000 pg/mL
- Seuil minimal de détection
- 31.25 pg/mL
- Application
- ELISA
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase 2, Mitochondrial (SOD2). No significant cross-reactivity or interference between Superoxide Dismutase 2, Mitochondrial (SOD2) and analogues was observed.
- Sensibilité
- 14.5 pg/mL
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- Discover our top product SOD2 Kit ELISA
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase 2, Mitochondrial (SOD2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutase 2, Mitochondrial (SOD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase 2, Mitochondrial (SOD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superoxide Dismutase 2, Mitochondrial (SOD2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase 2, Mitochondrial (SOD2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase 2, Mitochondrial (SOD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes SOD2 Kits ELISA
- SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))
- Autre désignation
- Superoxide Dismutase 2, Mitochondrial (SOD2 Produits)
- Synonymes
- LOC100101896 Kit ELISA, MNSOD Kit ELISA, GB14346 Kit ELISA, sod2 Kit ELISA, MGC88869 Kit ELISA, Sod2 Kit ELISA, CG8905 Kit ELISA, Dmel\\CG8905 Kit ELISA, Mito SOD Kit ELISA, Mn SOD Kit ELISA, Mn-SOD Kit ELISA, Mn-SOD2 Kit ELISA, MnSOD Kit ELISA, MnSODII Kit ELISA, Mn[2+]SOD Kit ELISA, SOD Kit ELISA, SOD-2 Kit ELISA, SOD2 Kit ELISA, Sod-2 Kit ELISA, dSOD2 Kit ELISA, mitSOD2 Kit ELISA, mnSOD Kit ELISA, IPOB Kit ELISA, MVCD6 Kit ELISA, cb463 Kit ELISA, wu:fj33b01 Kit ELISA, zgc:73051 Kit ELISA, MnSOD Kit ELISA, manganese superoxide dismutase Kit ELISA, superoxide dismutase 2, mitochondrial Kit ELISA, superoxide dismutase 2 Kit ELISA, Mn superoxide dismutase Kit ELISA, Superoxide dismutase 2 (Mn) Kit ELISA, superoxide dismutase 2 L homeolog Kit ELISA, BDBG_07234 Kit ELISA, LOC100101896 Kit ELISA, MNSOD Kit ELISA, Sod2 Kit ELISA, sod2 Kit ELISA, LbMnSOD4 Kit ELISA, SOD2 Kit ELISA, LOC100282741 Kit ELISA, SOD-2 Kit ELISA, sod2.L Kit ELISA
- Sujet
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Gene Name: Superoxide Dismutase 2, Mitochondrial
Gene Aliases: IPO-B, MNSOD, Mn-SOD
- UniProt
- P07895
- Pathways
- Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling
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