SUZ12 Kit ELISA

N° du produit ABIN5659017

Détail du produit SUZ12 Kit ELISA

Antigène: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Suppressor Of Zeste 12 Homolog (SUZ12). No significant cross-reactivity or interference between Suppressor Of Zeste 12 Homolog (SUZ12) and analogues was observed.

Sensibilité: 0.113 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Suppressor Of Zeste 12 Homolog (SUZ12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Suppressor Of Zeste 12 Homolog (SUZ12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Suppressor Of Zeste 12 Homolog (SUZ12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Suppressor Of Zeste 12 Homolog (SUZ12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Suppressor Of Zeste 12 Homolog (SUZ12) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Suppressor Of Zeste 12 Homolog (SUZ12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Autre désignation: Suppressor Of Zeste 12 Homolog (SUZ12 Produits)

Synonymes: fc31h10 Kit ELISA, im:7153578 Kit ELISA, si:dkey-10p5.2 Kit ELISA, suz12 Kit ELISA, wu:fc31h10 Kit ELISA, 2610028O16Rik Kit ELISA, AI195385 Kit ELISA, AU016842 Kit ELISA, AW536442 Kit ELISA, D11Ertd530e Kit ELISA, mKIAA0160 Kit ELISA, Xsuz12 Kit ELISA, chet9 Kit ELISA, jjaz1 Kit ELISA, CHET9 Kit ELISA, ChET 9 protein Kit ELISA, JJAZ1 Kit ELISA, suz12l Kit ELISA, zgc:100863 Kit ELISA, SUZ12 polycomb repressive complex 2b subunit Kit ELISA, SUZ12 polycomb repressive complex 2 subunit Kit ELISA, suppressor of zeste 12 homolog (Drosophila) Kit ELISA, SUZ12 polycomb repressive complex 2 subunit S homeolog Kit ELISA, SUZ12 polycomb repressive complex 2a subunit Kit ELISA, suz12b Kit ELISA, Suz12 Kit ELISA, SUZ12 Kit ELISA, suz12.S Kit ELISA, suz12a Kit ELISA

Sujet:

Gene Name: Suppressor Of Zeste 12 Homolog

Gene Aliases: CHET9, JJAZ1, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein