alpha Taxilin Kit ELISA

N° du produit ABIN5659102

Détail du produit alpha Taxilin Kit ELISA

Antigène: alpha Taxilin (TXLNA) (Taxilin alpha (TXLNA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Taxilin Alpha (TXLNa). No significant cross-reactivity or interference between Taxilin Alpha (TXLNa) and analogues was observed.

Sensibilité: 5.6 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Taxilin Alpha (TXLNa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Taxilin Alpha (TXLNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Taxilin Alpha (TXLNa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Taxilin Alpha (TXLNa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Taxilin Alpha (TXLNa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Taxilin Alpha (TXLNa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: alpha Taxilin (TXLNA) (Taxilin alpha (TXLNA))

Autre désignation: Taxilin Alpha (TXLNA Produits)

Synonymes: fc21b12 Kit ELISA, im:7039434 Kit ELISA, wu:fc21b12 Kit ELISA, zgc:162882 Kit ELISA, TXLNA Kit ELISA, txlnb Kit ELISA, MGC154810 Kit ELISA, 2600010N21Rik Kit ELISA, AI425952 Kit ELISA, AW209241 Kit ELISA, Il14 Kit ELISA, Txln Kit ELISA, IL14 Kit ELISA, RP4-622L5.4 Kit ELISA, TXLN Kit ELISA, taxilin alpha Kit ELISA, taxilin alpha S homeolog Kit ELISA, txlna Kit ELISA, TXLNA Kit ELISA, txlna.S Kit ELISA, MCYG_06155 Kit ELISA, Txlna Kit ELISA

Sujet:

Gene Name: Taxilin Alpha

Gene Aliases: IL14, TXLN, HMW-BCGF, High Molecular Weight B-Cell Growth Factor, Interleukin 14

ID gène: 200081

UniProt: P40222