TARS Kit ELISA

N° du produit ABIN5659159

Détail du produit TARS Kit ELISA

Antigène: TARS (threonyl-tRNA Synthetase (TARS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Threonyl tRNA Synthetase (TARS). No significant cross-reactivity or interference between Threonyl tRNA Synthetase (TARS) and analogues was observed.

Sensibilité: 0.115 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Threonyl tRNA Synthetase (TARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Threonyl tRNA Synthetase (TARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Threonyl tRNA Synthetase (TARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Threonyl tRNA Synthetase (TARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Threonyl tRNA Synthetase (TARS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Threonyl tRNA Synthetase (TARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TARS (threonyl-tRNA Synthetase (TARS))

Autre désignation: Threonyl tRNA Synthetase (TARS Produits)

Synonymes: ThrRS Kit ELISA, wu:fb07c05 Kit ELISA, wu:fb39c12 Kit ELISA, zgc:92586 Kit ELISA, 42/3 Kit ELISA, CG5353 Kit ELISA, Dmel\\CG5353 Kit ELISA, P539 Kit ELISA, TRS Kit ELISA, l(2)k04203 Kit ELISA, threonyl-tRNA synthetase Kit ELISA, tarsl2 Kit ELISA, TARS Kit ELISA, D15Wsu59e Kit ELISA, threonyl-tRNA synthetase L homeolog Kit ELISA, threonyl-tRNA synthetase Kit ELISA, Threonyl-tRNA synthetase Kit ELISA, threonyl-tRNA synthetase 2, mitochondrial (putative) Kit ELISA, tars.L Kit ELISA, tars Kit ELISA, TARS Kit ELISA, ThrRS Kit ELISA, thrS Kit ELISA, tars2 Kit ELISA, Tars Kit ELISA

Sujet:

Gene Name: Threonyl tRNA Synthetase

Gene Aliases: ThrRS, PL-7, PL7, Threonine tRNA Ligase 1,Cytoplasmic

ID gène: 6897

UniProt: P26639