Thymopoietin Kit ELISA

N° du produit ABIN5659214

Détail du produit Thymopoietin Kit ELISA

Antigène: Thymopoietin (TMPO)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.81 pg/mL - 500 pg/mL

Seuil minimal de détection: 7.81 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Thymopoietin (TMPO). No significant cross-reactivity or interference between Thymopoietin (TMPO) and analogues was observed.

Sensibilité: 12.9 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymopoietin (TMPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymopoietin (TMPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymopoietin (TMPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymopoietin (TMPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymopoietin (TMPO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymopoietin (TMPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Thymopoietin (TMPO)

Autre désignation: Thymopoietin (TMPO Produits)

Synonymes: LAP2 Kit ELISA, CMD1T Kit ELISA, LEMD4 Kit ELISA, PRO0868 Kit ELISA, TP Kit ELISA, lap2 Kit ELISA, LAP2beta Kit ELISA, 5630400D24Rik Kit ELISA, AI195756 Kit ELISA, AI606875 Kit ELISA, AW214352 Kit ELISA, AW547477 Kit ELISA, tmpo Kit ELISA, wu:fc26f12 Kit ELISA, wu:fd21f02 Kit ELISA, wu:fi29h05 Kit ELISA, thymopoietin Kit ELISA, thymopoietin S homeolog Kit ELISA, thymopoietin a Kit ELISA, TMPO Kit ELISA, Tmpo Kit ELISA, tmpo.S Kit ELISA, tmpoa Kit ELISA

Sujet:

Gene Name: Thymopoietin

Gene Aliases: TP, LAP2, CMD1T, LEMD4, TP5, Splenin, Lamina-Associated Polypeptide 2,Isoforms Beta/Gamma, LEM Domain Containing 4, Thymopoietin-related peptide isoforms beta/gamma

ID gène: 7112

UniProt: P42167