THRA Kit ELISA

N° du produit ABIN5659227

Détail du produit THRA Kit ELISA

Antigène: THRA (Thyroid Hormone Receptor, alpha (THRA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Thyroid Hormone Receptor Alpha (THRa). No significant cross-reactivity or interference between Thyroid Hormone Receptor Alpha (THRa) and analogues was observed.

Sensibilité: 22.5 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Hormone Receptor Alpha (THRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyroid Hormone Receptor Alpha (THRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Hormone Receptor Alpha (THRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Hormone Receptor Alpha (THRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Hormone Receptor Alpha (THRa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Hormone Receptor Alpha (THRa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: THRA (Thyroid Hormone Receptor, alpha (THRA))

Autre désignation: Thyroid Hormone Receptor Alpha (THRA Produits)

Synonymes: AR7 Kit ELISA, CHNG6 Kit ELISA, EAR7 Kit ELISA, ERB-T-1 Kit ELISA, ERBA Kit ELISA, ERBA1 Kit ELISA, NR1A1 Kit ELISA, THRA1 Kit ELISA, THRA2 Kit ELISA, c-ERBA-1 Kit ELISA, C-ERBA-ALPHA Kit ELISA, TRalpha Kit ELISA, c-erba Kit ELISA, erba Kit ELISA, nr1a1-A Kit ELISA, thra-A Kit ELISA, thra1 Kit ELISA, TR[a]1 Kit ELISA, nr1a1 Kit ELISA, thra Kit ELISA, thral Kit ELISA, tra Kit ELISA, tra1 Kit ELISA, wu:fb59g07 Kit ELISA, Thra1 Kit ELISA, 6430529J03Rik Kit ELISA, AW259572 Kit ELISA, Erba Kit ELISA, Nr1a1 Kit ELISA, Rvr Kit ELISA, T3R[a] Kit ELISA, T3Ralpha Kit ELISA, Thra2 Kit ELISA, C-ERBA ALPHA1 Kit ELISA, Thra Kit ELISA, fTHRalphaA Kit ELISA, nr1a1-a Kit ELISA, thyroid hormone receptor, alpha Kit ELISA, thyroid hormone receptor, alpha L homeolog Kit ELISA, thyroid hormone receptor alpha a Kit ELISA, thyroid hormone receptor alpha Kit ELISA, thyroid hormone receptor alpha-A Kit ELISA, THRA Kit ELISA, thra.L Kit ELISA, thraa Kit ELISA, Thra Kit ELISA, tr-alpha Kit ELISA, LOC109641414 Kit ELISA

Sujet:

Gene Name: Thyroid Hormone Receptor Alpha

Gene Aliases: THR-A, NR1A1, AR7, EAR7, ERB-T1, ERBA1, NR1-A1, THRA1, THRA2, c-ERBA1, Erythroblastic Leukemia Viral Oncogene Homolog, Nuclear Receptor Subfamily 1,Group A,Member 1

ID gène: 7067

UniProt: P10827

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Skeletal Muscle Fiber Development