TRAF3 Kit ELISA

N° du produit ABIN5659336

Détail du produit TRAF3 Kit ELISA

Antigène: TRAF3 (TNF Receptor-Associated Factor 3 (TRAF3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of TNF Receptor Associated Factor 3 (TRAF3). No significant cross-reactivity or interference between TNF Receptor Associated Factor 3 (TRAF3) and analogues was observed.

Sensibilité: 6.3 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF Receptor Associated Factor 3 (TRAF3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TNF Receptor Associated Factor 3 (TRAF3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF Receptor Associated Factor 3 (TRAF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNF Receptor Associated Factor 3 (TRAF3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNF Receptor Associated Factor 3 (TRAF3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNF Receptor Associated Factor 3 (TRAF3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TRAF3 (TNF Receptor-Associated Factor 3 (TRAF3))

Autre désignation: TNF Receptor Associated Factor 3 (TRAF3 Produits)

Synonymes: CAP-1 Kit ELISA, CAP1 Kit ELISA, CD40bp Kit ELISA, CRAF1 Kit ELISA, IIAE5 Kit ELISA, LAP1 Kit ELISA, AI528849 Kit ELISA, T-BAM Kit ELISA, amn Kit ELISA, TNF receptor associated factor 3 Kit ELISA, TNF receptor-associated factor 3 Kit ELISA, Tnf receptor-associated factor 3 Kit ELISA, TRAF3 Kit ELISA, Traf3 Kit ELISA

Sujet:

Gene Name: TNF Receptor Associated Factor 3

Gene Aliases: LAP1, CAP-1, CD40bp, CRAF1, CD40 receptor-associated factor 1, CD40-binding protein, LMP1-associated protein 1

Pathways: Signalisation NF-kappaB, Apoptose, Signalisation TLR, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades