TRAF6 Kit ELISA

N° du produit ABIN5659338

Détail du produit TRAF6 Kit ELISA

Antigène: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of TNF Receptor Associated Factor 6 (TRAF6). No significant cross-reactivity or interference between TNF Receptor Associated Factor 6 (TRAF6) and analogues was observed.

Sensibilité: 0.053 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF Receptor Associated Factor 6 (TRAF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TNF Receptor Associated Factor 6 (TRAF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF Receptor Associated Factor 6 (TRAF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNF Receptor Associated Factor 6 (TRAF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNF Receptor Associated Factor 6 (TRAF6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNF Receptor Associated Factor 6 (TRAF6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Autre désignation: TNF Receptor Associated Factor 6 (TRAF6 Produits)

Synonymes: MGC:3310 Kit ELISA, RNF85 Kit ELISA, 2310003F17Rik Kit ELISA, AI851288 Kit ELISA, C630032O20Rik Kit ELISA, rnf85 Kit ELISA, traf6 Kit ELISA, xtraf6 Kit ELISA, CG10961 Kit ELISA, DTRAF2 Kit ELISA, Dmel\\CG10961 Kit ELISA, TRAF Kit ELISA, TRAF2 Kit ELISA, TRAF6 Kit ELISA, Traf2 Kit ELISA, dTRAF2 Kit ELISA, dTRAF6 Kit ELISA, dTraf2 Kit ELISA, dtraf2 Kit ELISA, GB10539 Kit ELISA, fi15f02 Kit ELISA, wu:fi15f02 Kit ELISA, zgc:63704 Kit ELISA, TNF receptor associated factor 6 Kit ELISA, TNF receptor-associated factor 6 Kit ELISA, TNF receptor associated factor 6 L homeolog Kit ELISA, TNF-receptor-associated factor 6 Kit ELISA, TNF receptor associated factor 6 S homeolog Kit ELISA, TRAF6 Kit ELISA, Traf6 Kit ELISA, traf6.L Kit ELISA, traf6 Kit ELISA, LOC551819 Kit ELISA, traf6.S Kit ELISA

Sujet:

Gene Name: TNF Receptor Associated Factor 6

Gene Aliases: RNF85, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer

ID gène: 7189

UniProt: Q9Y4K3

Pathways: Signalisation NF-kappaB, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Hepatitis C, Toll-Like Receptors Cascades, Ubiquitin Proteasome Pathway