TOLLIP Kit ELISA

N° du produit ABIN5659345

Détail du produit TOLLIP Kit ELISA

Antigène: TOLLIP (Toll Interacting Protein (TOLLIP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Toll Interacting Protein (TOLLIP). No significant cross-reactivity or interference between Toll Interacting Protein (TOLLIP) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Interacting Protein (TOLLIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Interacting Protein (TOLLIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Interacting Protein (TOLLIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll Interacting Protein (TOLLIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Interacting Protein (TOLLIP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Interacting Protein (TOLLIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TOLLIP (Toll Interacting Protein (TOLLIP))

Autre désignation: Toll Interacting Protein (TOLLIP Produits)

Synonymes: IL-1RAcPIP Kit ELISA, 4930403G24Rik Kit ELISA, 4931428G15Rik Kit ELISA, fk22a02 Kit ELISA, im:7145391 Kit ELISA, wu:fk22a02 Kit ELISA, zgc:76985 Kit ELISA, TOLLIP Kit ELISA, GB17961 Kit ELISA, tollip Kit ELISA, tollip1 Kit ELISA, tollipa Kit ELISA, TOLIP Kit ELISA, toll interacting protein Kit ELISA, toll-interacting protein Kit ELISA, toll-interacting protein-like Kit ELISA, toll interacting protein L homeolog Kit ELISA, toll interacting protein S homeolog Kit ELISA, Toll-interleukine I receptor interacting protein I Kit ELISA, TOLLIP Kit ELISA, Tollip Kit ELISA, tollip Kit ELISA, LOC552034 Kit ELISA, LOC100169858 Kit ELISA, tollip.L Kit ELISA, tollip.S Kit ELISA, LOC100136122 Kit ELISA

Sujet:

Gene Name: Toll Interacting Protein

Gene Aliases: IL-1RAcPIP

ID gène: 54472

UniProt: Q9H0E2