Topoisomerase I Kit ELISA

N° du produit ABIN5659377

Détail du produit Topoisomerase I Kit ELISA

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Topoisomerase I (TOP1). No significant cross-reactivity or interference between Topoisomerase I (TOP1) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Topoisomerase I (TOP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Topoisomerase I (TOP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Topoisomerase I (TOP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Topoisomerase I (TOP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Topoisomerase I (TOP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Topoisomerase I (TOP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Autre désignation: Topoisomerase I (TOP1 Produits)

Synonymes: CG6146 Kit ELISA, Dmel\\CG6146 Kit ELISA, TOP1 Kit ELISA, TopI Kit ELISA, Topo I Kit ELISA, TopoI Kit ELISA, dTopoI Kit ELISA, l(1)G0134 Kit ELISA, l(1)G0201 Kit ELISA, l(1)G0229 Kit ELISA, l(1)G0278 Kit ELISA, l(1)top1 Kit ELISA, top1 Kit ELISA, topo I Kit ELISA, topoI Kit ELISA, DNA topoisomerase 1 beta Kit ELISA, DNA topoisomerase I alpha Kit ELISA, MGO1 Kit ELISA, MGOUN 1 Kit ELISA, MTE17.1 Kit ELISA, MTE17_1 Kit ELISA, TOP1BETA Kit ELISA, TOPOISOMERASE 1 Kit ELISA, TOPI Kit ELISA, AI467334 Kit ELISA, D130064I21Rik Kit ELISA, Top-1 Kit ELISA, Ab2-086 Kit ELISA, topi Kit ELISA, Topoisomerase 1 Kit ELISA, DNA topoisomerase I Kit ELISA, DNA topoisomerase I alpha Kit ELISA, DNA topoisomerase 1 Kit ELISA, topoisomerase (DNA) I Kit ELISA, DNA topoisomerase I, gene 1 L homeolog Kit ELISA, Top1 Kit ELISA, top1 Kit ELISA, topA Kit ELISA, ZMO1193 Kit ELISA, MRAD2831_RS63095 Kit ELISA, Lferr_0016 Kit ELISA, PC1_2015 Kit ELISA, Za10_0136 Kit ELISA, Gbro_0575 Kit ELISA, Dd586_2108 Kit ELISA, Kvar_3044 Kit ELISA, Alvin_2069 Kit ELISA, Dacet_0849 Kit ELISA, Mrub_1615 Kit ELISA, Arnit_2962 Kit ELISA, Ndas_4953 Kit ELISA, Trad_2377 Kit ELISA, Acear_1653 Kit ELISA, Mahau_0844 Kit ELISA, Mesop_4109 Kit ELISA, Thein_1586 Kit ELISA, Theth_0241 Kit ELISA, TOP1ALPHA Kit ELISA, top-1 Kit ELISA, TOP1 Kit ELISA, top1.1.L Kit ELISA

Classe de substances: Viral Protein

Sujet:

Gene Name: Topoisomerase I

Gene Aliases: TOPI, DNA Topoisomerase 1

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance