TOP2 Kit ELISA

N° du produit ABIN5659381

Détail du produit TOP2 Kit ELISA

Antigène: TOP2 (DNA Topoisomerase II (TOP2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Topoisomerase II (TOP2). No significant cross-reactivity or interference between Topoisomerase II (TOP2) and analogues was observed.

Sensibilité: 0.061 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Topoisomerase II (TOP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Topoisomerase II (TOP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Topoisomerase II (TOP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Topoisomerase II (TOP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Topoisomerase II (TOP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Topoisomerase II (TOP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TOP2 (DNA Topoisomerase II (TOP2))

Autre désignation: Topoisomerase II (TOP2 Produits)

Synonymes: Topoii Kit ELISA, ptr11 Kit ELISA, DDBDRAFT_0191045 Kit ELISA, DDBDRAFT_0231510 Kit ELISA, DDB_0191045 Kit ELISA, DDB_0231510 Kit ELISA, CG10223 Kit ELISA, CHRAC Kit ELISA, Dmel\\CG10223 Kit ELISA, TOP2 Kit ELISA, TOPO II Kit ELISA, TOPOII Kit ELISA, TopII Kit ELISA, Topo II Kit ELISA, TopoII Kit ELISA, dCHRAC Kit ELISA, dTopoII Kit ELISA, top2 Kit ELISA, topo II Kit ELISA, topoII Kit ELISA, topoisomerase II Kit ELISA, DNA topoisomerase II Kit ELISA, DNA topoisomerase 2 Kit ELISA, DNA topoisomerase ii Kit ELISA, type IIA topoisomerase; DNA gyrase topoisomerase IV B subunit Kit ELISA, Topoisomerase 2 Kit ELISA, TOP2 Kit ELISA, A583L Kit ELISA, top2 Kit ELISA, CNA03600 Kit ELISA, Tb09.160.4090 Kit ELISA, Tb11.01.3390 Kit ELISA, LMJF_28_2280 Kit ELISA, Top2 Kit ELISA

Sujet:

Gene Name: Topoisomerase II

Gene Aliases: DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme

ID gène: 360243

UniProt: P41516

Pathways: Mitotic G1-G1/S Phases