TFAM Kit ELISA

N° du produit ABIN5659393

Détail du produit TFAM Kit ELISA

Antigène: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Transcription Factor A, Mitochondrial (TFAM). No significant cross-reactivity or interference between Transcription Factor A, Mitochondrial (TFAM) and analogues was observed.

Sensibilité: 0.124 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription Factor A, Mitochondrial (TFAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcription Factor A, Mitochondrial (TFAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcription Factor A, Mitochondrial (TFAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transcription Factor A, Mitochondrial (TFAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transcription Factor A, Mitochondrial (TFAM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transcription Factor A, Mitochondrial (TFAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Autre désignation: Transcription Factor A, Mitochondrial (TFAM Produits)

Synonymes: tcf6 Kit ELISA, mttf1 Kit ELISA, mttfa Kit ELISA, tcf6l1 Kit ELISA, tcf6l2 Kit ELISA, tcf6l3 Kit ELISA, mttfa-A Kit ELISA, xl-mtTFA Kit ELISA, MGC153358 Kit ELISA, zgc:153358 Kit ELISA, MTTF1 Kit ELISA, MTTFA Kit ELISA, TCF6 Kit ELISA, TCF6L1 Kit ELISA, TCF6L2 Kit ELISA, TCF6L3 Kit ELISA, AI661103 Kit ELISA, Hmgts Kit ELISA, mtTFA Kit ELISA, tsHMG Kit ELISA, Mttfa Kit ELISA, transcription factor A, mitochondrial Kit ELISA, transcription factor A, mitochondrial L homeolog Kit ELISA, TFAM Kit ELISA, tfam.L Kit ELISA, tfam Kit ELISA, Tfam Kit ELISA

Sujet:

Gene Name: Transcription Factor A, Mitochondrial

Gene Aliases: MtTF1, TCF6, TCF6L2, mtTFA, Mitochondrial transcription factor 1, Transcription factor 6-like 2

ID gène: 7019

UniProt: Q00059

Pathways: Chromatin Binding