TFE3 Kit ELISA

N° du produit ABIN5659395

Détail du produit TFE3 Kit ELISA

Antigène: TFE3 (Transcription Factor E3 (TFE3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Transcription Factor Binding To IGHM Enhancer 3 (TFE3). No significant cross-reactivity or interference between Transcription Factor Binding To IGHM Enhancer 3 (TFE3) and analogues was observed.

Sensibilité: 0.118 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription Factor Binding To IGHM Enhancer 3 (TFE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcription Factor Binding To IGHM Enhancer 3 (TFE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcription Factor Binding To IGHM Enhancer 3 (TFE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transcription Factor Binding To IGHM Enhancer 3 (TFE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transcription Factor Binding To IGHM Enhancer 3 (TFE3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transcription Factor Binding To IGHM Enhancer 3 (TFE3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TFE3 (Transcription Factor E3 (TFE3))

Autre désignation: Transcription Factor Binding To IGHM Enhancer 3 (TFE3 Produits)

Synonymes: RCCP2 Kit ELISA, RCCX1 Kit ELISA, TFEA Kit ELISA, bHLHe33 Kit ELISA, F830016E06Rik Kit ELISA, Tcfe3 Kit ELISA, Tfe-3 Kit ELISA, RGD1559642 Kit ELISA, cb873 Kit ELISA, zgc:109815 Kit ELISA, transcription factor binding to IGHM enhancer 3 Kit ELISA, transcription factor E3 Kit ELISA, transcription factor binding to IGHM enhancer 3 S homeolog Kit ELISA, transcription factor binding to IGHM enhancer 3a Kit ELISA, TFE3 Kit ELISA, Tfe3 Kit ELISA, tfe3.S Kit ELISA, tfe3a Kit ELISA

Sujet:

Gene Name: Transcription Factor Binding To IGHM Enhancer 3

Gene Aliases: RCCP2, TFEA, BHLHE33, Transcription Tactor E Family Member A, Class E basic helix-loop-helix protein 33, Transcription factor E3