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TNNI1 Kit ELISA

TNNI1 Reactivité: Humain Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5659554
  • Antigène Voir toutes TNNI1 Kits ELISA
    TNNI1 (Slow Skeletal Troponin I (TNNI1))
    Reactivité
    • 4
    • 2
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    46.88 pg/mL - 3000 pg/mL
    Seuil minimal de détection
    46.88 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Troponin I Type 1, Slow Skeletal (TNNI1). No significant cross-reactivity or interference between Troponin I Type 1, Slow Skeletal (TNNI1) and analogues was observed.
    Sensibilité
    17.59 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin I Type 1, Slow Skeletal (TNNI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin I Type 1, Slow Skeletal (TNNI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin I Type 1, Slow Skeletal (TNNI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin I Type 1, Slow Skeletal (TNNI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin I Type 1, Slow Skeletal (TNNI1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin I Type 1, Slow Skeletal (TNNI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes TNNI1 Kits ELISA
    TNNI1 (Slow Skeletal Troponin I (TNNI1))
    Autre désignation
    Troponin I Type 1, Slow Skeletal (TNNI1 Produits)
    Synonymes
    ANM Kit ELISA, NEM5 Kit ELISA, STNT Kit ELISA, TNT Kit ELISA, TNTS Kit ELISA, TnI-S4 Kit ELISA, SSTNI Kit ELISA, TNN1 Kit ELISA, 2700018B22Rik Kit ELISA, AI747285 Kit ELISA, ssTnI Kit ELISA, AW146156 Kit ELISA, Tnt Kit ELISA, sTnT Kit ELISA, ssTnT Kit ELISA, Fang2 Kit ELISA, tnTs Kit ELISA, troponin 1 Kit ELISA, zgc:86895 Kit ELISA, troponin T1, slow skeletal type Kit ELISA, troponin I1, slow skeletal type Kit ELISA, troponin I, skeletal, slow 1 Kit ELISA, troponin T1, skeletal, slow Kit ELISA, troponin I1, slow skeletal type S homeolog Kit ELISA, troponin I type 1 (skeletal, slow) Kit ELISA, troponin I4b, tandem duplicate 2 Kit ELISA, TNNT1 Kit ELISA, TNNI1 Kit ELISA, Tnni1 Kit ELISA, Tnnt1 Kit ELISA, tnni1.S Kit ELISA, tnni4b.2 Kit ELISA
    Sujet

    Gene Name: Troponin I Type 1, Slow Skeletal

    Gene Aliases: TNN1, Troponin I, slow-twitch isoform

    ID gène
    7135
    UniProt
    P19237
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