TPSAB1 Kit ELISA

N° du produit ABIN5659587

Détail du produit TPSAB1 Kit ELISA

Antigène: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8 pg/mL - 500 pg/mL

Seuil minimal de détection: 7.8 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Tryptase (TPS). No significant cross-reactivity or interference between Tryptase (TPS) and analogues was observed.

Sensibilité: 3.1 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptase (TPS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tryptase (TPS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tryptase (TPS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tryptase (TPS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tryptase (TPS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tryptase (TPS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Autre désignation: Tryptase (TPSAB1 Produits)

Synonymes: TPS1 Kit ELISA, TPS2 Kit ELISA, TPSB1 Kit ELISA, PMCT7 Kit ELISA, tpsab1 Kit ELISA, tryptaseB Kit ELISA, tryptaseC Kit ELISA, MMCP-7 Kit ELISA, Mcp-7 Kit ELISA, Mcp7 Kit ELISA, Mcpt7 Kit ELISA, rMCP-7 Kit ELISA, AV011504 Kit ELISA, MMCP-6 Kit ELISA, Mcp-6 Kit ELISA, Mcp6 Kit ELISA, Mcpt6 Kit ELISA, tryptase alpha/beta 1 Kit ELISA, tryptase Kit ELISA, tryptase beta 2 (gene/pseudogene) Kit ELISA, tryptase beta 2 Kit ELISA, TPSAB1 Kit ELISA, MCT7 Kit ELISA, tpsab1 Kit ELISA, TPSB2 Kit ELISA, Tpsab1 Kit ELISA, Tpsb2 Kit ELISA

Sujet:

Gene Name: Tryptase

Gene Aliases: TPSAB1, Tryptase Alpha/Beta 1, Tryptase Alpha II, Tryptase Beta I, Tryptase-I, Tryptase-II, Tryptase-III

UniProt: Q15661