TDO2 Kit ELISA

N° du produit ABIN5659599

Détail du produit TDO2 Kit ELISA

Antigène: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Tryptophan-2,3-dioxygenase (TDO). No significant cross-reactivity or interference between Tryptophan-2,3-dioxygenase (TDO) and analogues was observed.

Sensibilité: 0.061 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptophan-2,3-dioxygenase (TDO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tryptophan-2,3-dioxygenase (TDO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tryptophan-2,3-dioxygenase (TDO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tryptophan-2,3-dioxygenase (TDO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tryptophan-2,3-dioxygenase (TDO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tryptophan-2,3-dioxygenase (TDO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Autre désignation: Tryptophan-2,3-dioxygenase (TDO2 Produits)

Synonymes: Tdo2 Kit ELISA, tdo2l Kit ELISA, fi30d08 Kit ELISA, zgc:63488 Kit ELISA, wu:fi30d08 Kit ELISA, TDO2 Kit ELISA, fb62b10 Kit ELISA, tdo2 Kit ELISA, wu:fb62b10 Kit ELISA, zgc:103693 Kit ELISA, DDBDRAFT_0190495 Kit ELISA, DDBDRAFT_0231363 Kit ELISA, DDB_0190495 Kit ELISA, DDB_0231363 Kit ELISA, TDO Kit ELISA, TO Kit ELISA, TPH2 Kit ELISA, TRPO Kit ELISA, AA407491 Kit ELISA, chky Kit ELISA, Tdo Kit ELISA, tryptophan 2,3-dioxygenase b Kit ELISA, tryptophan 2,3-dioxygenase Kit ELISA, tryptophan 2,3-dioxygenase a Kit ELISA, tryptophan 2,3-dioxygenase L homeolog Kit ELISA, tdo2b Kit ELISA, tdo2 Kit ELISA, TDO2 Kit ELISA, MADE_02822 Kit ELISA, CtCNB1_3657 Kit ELISA, tdo2a Kit ELISA, CNC04830 Kit ELISA, tdo Kit ELISA, Mrub_2119 Kit ELISA, Deipr_2235 Kit ELISA, Acav_1166 Kit ELISA, Mesop_4278 Kit ELISA, Tdo2 Kit ELISA, tdo2.L Kit ELISA

Sujet:

Gene Name: Tryptophan-2,3-dioxygenase

Gene Aliases: TDO2, TPH2, TO, TRPO, Tryptophanase, Tryptophan oxygenase

ID gène: 6999

UniProt: P48775