TUBA4A Kit ELISA

N° du produit ABIN5659602

Détail du produit TUBA4A Kit ELISA

Antigène: TUBA4A (Tubulin, alpha 4a (TUBA4A))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Tubulin Alpha 4A (TUBa4A). No significant cross-reactivity or interference between Tubulin Alpha 4A (TUBa4A) and analogues was observed.

Sensibilité: 0.124 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tubulin Alpha 4A (TUBa4A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tubulin Alpha 4A (TUBa4A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tubulin Alpha 4A (TUBa4A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tubulin Alpha 4A (TUBa4A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tubulin Alpha 4A (TUBa4A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tubulin Alpha 4A (TUBa4A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TUBA4A (Tubulin, alpha 4a (TUBA4A))

Autre désignation: Tubulin Alpha 4A (TUBA4A Produits)

Synonymes: TUBA1 Kit ELISA, TUBA6 Kit ELISA, H2-ALPHA Kit ELISA, M[a]4 Kit ELISA, Tuba4 Kit ELISA, tuba1 Kit ELISA, tubulin alpha 1a Kit ELISA, tubulin alpha 4a Kit ELISA, tubulin, alpha 4A Kit ELISA, tubulin alpha 4a L homeolog Kit ELISA, TUBA1A Kit ELISA, TUBA4A Kit ELISA, Tuba4a Kit ELISA, tuba4a.L Kit ELISA

Sujet:

Gene Name: Tubulin Alpha 4A

Gene Aliases: TUBA1, Testis Specific Tubulin Alpha 1, Alpha-tubulin 1, Tubulin alpha-1 chain, Tubulin H2-alpha

Pathways: Dynamique des Microtubules, M Phase