TNFAIP3 Kit ELISA

N° du produit ABIN5659643

Détail du produit TNFAIP3 Kit ELISA

Antigène: TNFAIP3 (Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.12 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3). No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3) and analogues was observed.

Sensibilité: 136 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TNFAIP3 (Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3))

Autre désignation: Tumor Necrosis Factor Alpha Induced Protein 3 (TNFAIP3 Produits)

Synonymes: tnfaip3 Kit ELISA, MGC82339 Kit ELISA, TNFAIP3 Kit ELISA, A20 Kit ELISA, OTUD7C Kit ELISA, TNFA1P2 Kit ELISA, Tnfip3 Kit ELISA, TNF alpha induced protein 3 Kit ELISA, TNF alpha induced protein 3 S homeolog Kit ELISA, tumor necrosis factor, alpha-induced protein 3 Kit ELISA, TNFAIP3 Kit ELISA, tnfaip3.S Kit ELISA, Tnfaip3 Kit ELISA

Sujet:

Gene Name: Tumor Necrosis Factor Alpha Induced Protein 3

Gene Aliases: A20, OTUD7C, TNFA1P2, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20

ID gène: 7128

UniProt: P21580

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Production of Molecular Mediator of Immune Response