TPT1 Kit ELISA

N° du produit ABIN5659696

Détail du produit TPT1 Kit ELISA

Antigène: TPT1 (Tumor Protein, Translationally-Controlled 1 (TPT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Tumor Protein, Translationally Controlled 1 (TPT1). No significant cross-reactivity or interference between Tumor Protein, Translationally Controlled 1 (TPT1) and analogues was observed.

Sensibilité: 0.128 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Protein, Translationally Controlled 1 (TPT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Protein, Translationally Controlled 1 (TPT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Protein, Translationally Controlled 1 (TPT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Protein, Translationally Controlled 1 (TPT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Protein, Translationally Controlled 1 (TPT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Protein, Translationally Controlled 1 (TPT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TPT1 (Tumor Protein, Translationally-Controlled 1 (TPT1))

Autre désignation: Tumor Protein, Translationally Controlled 1 (TPT1 Produits)

Synonymes: HRF Kit ELISA, TCTP Kit ELISA, p02 Kit ELISA, Trt Kit ELISA, p21 Kit ELISA, p23 Kit ELISA, TPT1 Kit ELISA, TCT1 Kit ELISA, tctp Kit ELISA, fa28a02 Kit ELISA, id:ibd2078 Kit ELISA, wu:fa28a02 Kit ELISA, PCHK23 Kit ELISA, hrf Kit ELISA, MGC79720 Kit ELISA, Tpt1 Kit ELISA, translationally controlled tumor protein Kit ELISA, CG4800 Kit ELISA, Dmel\\CG4800 Kit ELISA, dTCTP Kit ELISA, Tctp Kit ELISA, tumor protein, translationally-controlled 1 Kit ELISA, tumor protein, translationally-controlled 1 L homeolog Kit ELISA, translationally-controlled tumor protein Kit ELISA, translationally controlled tumor protein Kit ELISA, translationally controlled tumor-like protein Kit ELISA, Translationally controlled tumor protein Kit ELISA, translationally-controlled tumor protein homolog Kit ELISA, P23 tumor protein-like Kit ELISA, TPT1 Kit ELISA, Tpt1 Kit ELISA, tpt1 Kit ELISA, tpt1.L Kit ELISA, LOC711798 Kit ELISA, TCTP Kit ELISA, LOC548038 Kit ELISA, Tctp Kit ELISA, LOC108708523 Kit ELISA, LOC109404863 Kit ELISA, LOC105389397 Kit ELISA, LOC102577554 Kit ELISA

Sujet:

Gene Name: Tumor Protein, Translationally Controlled 1

Gene Aliases: HRF, TCTP, p02, p23, Fortilin, Translationally-Controlled Tumor Protein, Histamine-releasing factor

ID gène: 7178

UniProt: P13693

Pathways: Response to Water Deprivation, Regulation of Cell Size, Negative Regulation of intrinsic apoptotic Signaling