TSG101 Kit ELISA

N° du produit ABIN5659699

Détail du produit TSG101 Kit ELISA

Antigène: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Tumor Susceptibility Gene 101 (TSG101). No significant cross-reactivity or interference between Tumor Susceptibility Gene 101 (TSG101) and analogues was observed.

Sensibilité: 0.114 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Susceptibility Gene 101 (TSG101). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Susceptibility Gene 101 (TSG101). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Susceptibility Gene 101 (TSG101), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Susceptibility Gene 101 (TSG101) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Susceptibility Gene 101 (TSG101) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Susceptibility Gene 101 (TSG101) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Autre désignation: Tumor Susceptibility Gene 101 (TSG101 Produits)

Synonymes: CG9712 Kit ELISA, DmTSG101 Kit ELISA, Dmel\\CG9712 Kit ELISA, ESCRT-I Kit ELISA, Tsg101 Kit ELISA, burs Kit ELISA, dTSg101 Kit ELISA, dTsg101 Kit ELISA, dVps23 Kit ELISA, ept Kit ELISA, tsg101 Kit ELISA, vps23 Kit ELISA, TSG101 Kit ELISA, MGC76193 Kit ELISA, TSG10 Kit ELISA, VPS23 Kit ELISA, AI255943 Kit ELISA, CC2 Kit ELISA, Rw Kit ELISA, zgc:86926 Kit ELISA, tumor susceptibility 101 Kit ELISA, Tumor susceptibility gene 101 Kit ELISA, Tumor Susceptibility Gene homolog Kit ELISA, tumor susceptibility gene 101 Kit ELISA, tumor susceptibility 101a Kit ELISA, tumor susceptibility 101 L homeolog Kit ELISA, TSG101 Kit ELISA, tsg-101 Kit ELISA, tsg101 Kit ELISA, Tsg101 Kit ELISA, tsg101a Kit ELISA, tsg101.L Kit ELISA

Sujet:

Gene Name: Tumor Susceptibility Gene 101

Gene Aliases: TSG10, VPS23, Tumor Susceptibility Gene 10, ESCRT-I complex subunit TSG101