TWIST1 Kit ELISA

N° du produit ABIN5659702

Détail du produit TWIST1 Kit ELISA

Antigène: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Twist Transcription Factor (TWIST). No significant cross-reactivity or interference between Twist Transcription Factor (TWIST) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Twist Transcription Factor (TWIST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Twist Transcription Factor (TWIST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Twist Transcription Factor (TWIST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Twist Transcription Factor (TWIST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Twist Transcription Factor (TWIST) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Twist Transcription Factor (TWIST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Autre désignation: Twist Transcription Factor (TWIST1 Produits)

Synonymes: scs Kit ELISA, Xtwi Kit ELISA, acs3 Kit ELISA, X-twi Kit ELISA, bpes2 Kit ELISA, bpes3 Kit ELISA, twist Kit ELISA, Xtwist Kit ELISA, MGC69439 Kit ELISA, twist1 Kit ELISA, ACS3 Kit ELISA, BPES2 Kit ELISA, BPES3 Kit ELISA, CRS1 Kit ELISA, SCS Kit ELISA, TWIST Kit ELISA, bHLHa38 Kit ELISA, AA960487 Kit ELISA, M-Twist Kit ELISA, Pde Kit ELISA, Ska10 Kit ELISA, Ska Kit ELISA, Twist Kit ELISA, pdt Kit ELISA, cTwist Kit ELISA, Twist1b Kit ELISA, twist-1 Kit ELISA, wu:fd04c01 Kit ELISA, zgc:101115 Kit ELISA, twist family bHLH transcription factor 1 Kit ELISA, twist family bHLH transcription factor 1 S homeolog Kit ELISA, twist basic helix-loop-helix transcription factor 1 Kit ELISA, twist homolog 1 (Drosophila) Kit ELISA, twist family bHLH transcription factor 1a Kit ELISA, TWIST1 Kit ELISA, twist1 Kit ELISA, twist1.S Kit ELISA, Twist1 Kit ELISA, twist1a Kit ELISA

Sujet:

Gene Name: Twist Transcription Factor

Gene Aliases: ACS3, BPES2, BPES3, SCS, Class A basic helix-loop-helix protein 38, Acrocephalosyndactyly 3, Saethre-Chotzen Syndrome, Blepharophimosis,Epicanthus Inversus And Ptosis 3

Pathways: Signalisation p53, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity