TWSG1 Kit ELISA

N° du produit ABIN5659703

Détail du produit TWSG1 Kit ELISA

Antigène: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Twisted Gastrulation Protein Homolog 1 (TWSG1). No significant cross-reactivity or interference between Twisted Gastrulation Protein Homolog 1 (TWSG1) and analogues was observed.

Sensibilité: 0.122 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Twisted Gastrulation Protein Homolog 1 (TWSG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Twisted Gastrulation Protein Homolog 1 (TWSG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Twisted Gastrulation Protein Homolog 1 (TWSG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Twisted Gastrulation Protein Homolog 1 (TWSG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Twisted Gastrulation Protein Homolog 1 (TWSG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Twisted Gastrulation Protein Homolog 1 (TWSG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Autre désignation: Twisted Gastrulation Protein Homolog 1 (TWSG1 Produits)

Synonymes: TWSG1 Kit ELISA, TSG Kit ELISA, DKFZp469J051 Kit ELISA, 1810013J15Rik Kit ELISA, 9030422N06Rik Kit ELISA, AW552143 Kit ELISA, D17Ertd403e Kit ELISA, Tsg Kit ELISA, Twg Kit ELISA, fi23f01 Kit ELISA, tsg Kit ELISA, tsga Kit ELISA, twsg1 Kit ELISA, wu:fi23f01 Kit ELISA, twsg Kit ELISA, tsg1 Kit ELISA, tsgb Kit ELISA, ztsg1 Kit ELISA, twisted gastrulation BMP signaling modulator 1 Kit ELISA, twisted gastrulation homolog 1 (Drosophila) Kit ELISA, twisted gastrulation BMP signaling modulator 1a Kit ELISA, twisted gastrulation BMP signaling modulator 1 S homeolog Kit ELISA, twisted gastrulation BMP signaling modulator 1 L homeolog Kit ELISA, twisted gastrulation BMP signaling modulator 1b Kit ELISA, TWSG1 Kit ELISA, Twsg1 Kit ELISA, twsg1a Kit ELISA, twsg1.S Kit ELISA, twsg1.L Kit ELISA, twsg1b Kit ELISA

Sujet:

Gene Name: Twisted Gastrulation Protein Homolog 1

Gene Aliases: TSG

ID gène: 57045

UniProt: Q9GZX9