UQCRC2 Kit ELISA

N° du produit ABIN5659722

Détail du produit UQCRC2 Kit ELISA

Antigène: UQCRC2 (Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2). No significant cross-reactivity or interference between Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) and analogues was observed.

Sensibilité: 0.061 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: UQCRC2 (Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2))

Autre désignation: Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2 Produits)

Synonymes: MC3DN5 Kit ELISA, QCR2 Kit ELISA, UQCR2 Kit ELISA, fi06a11 Kit ELISA, uqcrc2 Kit ELISA, wu:fb64g04 Kit ELISA, wu:fi06a11 Kit ELISA, fb33d01 Kit ELISA, fc43c05 Kit ELISA, wu:fb33d01 Kit ELISA, wu:fc43c05 Kit ELISA, zgc:92453 Kit ELISA, MGC80228 Kit ELISA, MGC130698 Kit ELISA, Uqcrc2 Kit ELISA, Afu5g04210 Kit ELISA, AO090102000625 Kit ELISA, 1500004O06Rik Kit ELISA, UQCRC2 Kit ELISA, ubiquinol-cytochrome c reductase core protein 2 Kit ELISA, ubiquinol cytochrome c reductase core protein 2 Kit ELISA, ubiquinol-cytochrome c reductase core protein 2b Kit ELISA, ubiquinol-cytochrome c reductase core protein II Kit ELISA, ubiquinol-cytochrome c reductase core protein 2a Kit ELISA, ubiquinol-cytochrome c reductase core protein 2 L homeolog Kit ELISA, cytochrome b-c1 complex subunit 2, mitochondrial Kit ELISA, ubiquinol-cytochrome C reductase complex core protein 2 Kit ELISA, ubiquinol--cytochrome-c reductase subunit 2 Kit ELISA, UQCRC2 Kit ELISA, Uqcrc2 Kit ELISA, uqcrc2b Kit ELISA, uqcrc2a Kit ELISA, uqcrc2.L Kit ELISA, uqcrc2 Kit ELISA, LOC100026672 Kit ELISA, LOC100389097 Kit ELISA, LOC100456655 Kit ELISA, LOC100592631 Kit ELISA, AFUA_5G04210 Kit ELISA, ANI_1_872084 Kit ELISA, AOR_1_1048134 Kit ELISA, QCR2 Kit ELISA, LOC100861263 Kit ELISA

Sujet:

Gene Name: Ubiquinol Cytochrome C Reductase Core Protein II

Gene Aliases: QCR2, UQCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Complex III subunit 2, Core protein II