UBA52 Kit ELISA

N° du produit ABIN5659724

Détail du produit UBA52 Kit ELISA

Antigène: UBA52 (Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1 (UBA52))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52). No significant cross-reactivity or interference between Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) and analogues was observed.

Sensibilité: 0.116 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: UBA52 (Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1 (UBA52))

Autre désignation: Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52 Produits)

Synonymes: CEP52 Kit ELISA, HUBCEP52 Kit ELISA, L40 Kit ELISA, RPL40 Kit ELISA, D8Ertd21e Kit ELISA, Gm1863 Kit ELISA, Rps27a Kit ELISA, Ubb Kit ELISA, Ubc Kit ELISA, UBCEP2 Kit ELISA, UB-RPL40 Kit ELISA, MGC80109 Kit ELISA, cep52 Kit ELISA, hubcep52 Kit ELISA, rpl40 Kit ELISA, RPS27A Kit ELISA, UBB Kit ELISA, UBC Kit ELISA, wu:fa91f08 Kit ELISA, zgc:123344 Kit ELISA, UBA52 Kit ELISA, ubiquitin A-52 residue ribosomal protein fusion product 1 Kit ELISA, ubiquitin A-52 residue ribosomal protein fusion product 1 L homeolog Kit ELISA, UBA52 Kit ELISA, Uba52 Kit ELISA, uba52.L Kit ELISA, uba52 Kit ELISA

Sujet:

Gene Name: Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1

Gene Aliases: CEP52, HUBCEP52, RPL40, 60S Ribosomal Protein L40, Ubiquitin Carboxyl Extension Protein 52

ID gène: 7311

UniProt: P62987

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, EGFR Downregulation