UBAP2 Kit ELISA

N° du produit ABIN5659725

Détail du produit UBAP2 Kit ELISA

Antigène: UBAP2 (Ubiquitin Associated Protein 2 (UBAP2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ubiquitin Associated Protein 2 (UBAP2). No significant cross-reactivity or interference between Ubiquitin Associated Protein 2 (UBAP2) and analogues was observed.

Sensibilité: 0.136 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Associated Protein 2 (UBAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Associated Protein 2 (UBAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Associated Protein 2 (UBAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Associated Protein 2 (UBAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Associated Protein 2 (UBAP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Associated Protein 2 (UBAP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: UBAP2 (Ubiquitin Associated Protein 2 (UBAP2))

Autre désignation: Ubiquitin Associated Protein 2 (UBAP2 Produits)

Synonymes: UBAP-2 Kit ELISA, bA176F3.5 Kit ELISA, AD012W Kit ELISA, zgc:158449 Kit ELISA, MGC132149 Kit ELISA, 1190005K07Rik Kit ELISA, AA408600 Kit ELISA, AU045235 Kit ELISA, mKIAA1491 Kit ELISA, UBAP2Z Kit ELISA, ubiquitin associated protein 2 Kit ELISA, ubiquitin associated protein 2a Kit ELISA, ubiquitin associated protein 2 L homeolog Kit ELISA, ubiquitin-associated protein 2 Kit ELISA, AD012Z Kit ELISA, UBAP2 Kit ELISA, ubap2a Kit ELISA, ubap2.L Kit ELISA, LOC100303669 Kit ELISA, ubap2 Kit ELISA, Ubap2 Kit ELISA, LOC407092 Kit ELISA

Sujet:

Gene Name: Ubiquitin Associated Protein 2

ID gène: 55833

UniProt: Q5T6F2

Pathways: SARS-CoV-2 Protein Interactome