UBE2L3 Kit CLIA

N° du produit ABIN5659736

Détail du produit UBE2L3 Kit CLIA

Antigène: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). No significant cross-reactivity or interference between Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) and analogues was observed.

Sensibilité: 5.28 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Autre désignation: Ubiquitin Conjugating Enzyme E2L3 (UBE2L3 Produits)

Synonymes: E2-F1 Kit CLIA, L-UBC Kit CLIA, UBCH7 Kit CLIA, UbcM4 Kit CLIA, C79827 Kit CLIA, Ubce7 Kit CLIA, ube2l3 Kit CLIA, wu:fc22h11 Kit CLIA, zgc:86727 Kit CLIA, UBE2L3 Kit CLIA, ube2l3l Kit CLIA, wu:fb38h03 Kit CLIA, ubiquitin conjugating enzyme E2 L3 Kit CLIA, ubiquitin-conjugating enzyme E2L 3 Kit CLIA, ubiquitin-conjugating enzyme E2L 3a Kit CLIA, ubiquitin conjugating enzyme E2 L3 S homeolog Kit CLIA, ubiquitin-conjugating enzyme E2L 3b Kit CLIA, UBE2L3 Kit CLIA, Ube2l3 Kit CLIA, ube2l3a Kit CLIA, ube2l3.S Kit CLIA, ube2l3b Kit CLIA

Sujet:

Gene Name: Ubiquitin Conjugating Enzyme E2L3

Gene Aliases: L-UBC, E2-F1, UBCH7, UbcM4, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3

ID gène: 7332

UniProt: P68036