ISG15 Kit CLIA

N° du produit ABIN5659738

Détail du produit ISG15 Kit CLIA

Antigène: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 312.5 pg/mL - 20000 pg/mL

Seuil minimal de détection: 312.5 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ubiquitin Cross Reactive Protein (UCRP). No significant cross-reactivity or interference between Ubiquitin Cross Reactive Protein (UCRP) and analogues was observed.

Sensibilité: 11.51 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Cross Reactive Protein (UCRP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ubiquitin Cross Reactive Protein (UCRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ubiquitin Cross Reactive Protein (UCRP) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Cross Reactive Protein (UCRP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Cross Reactive Protein (UCRP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Autre désignation: Ubiquitin Cross Reactive Protein (ISG15 Produits)

Synonymes: G1P2 Kit CLIA, IFI15 Kit CLIA, IP17 Kit CLIA, UCRP Kit CLIA, hUCRP Kit CLIA, ISG15 Kit CLIA, g1p2 Kit CLIA, si:rp71-1c23.2 Kit CLIA, G1p2 Kit CLIA, IGI15 Kit CLIA, Irfp Kit CLIA, ISG17 Kit CLIA, ISG17/UCRP Kit CLIA, ISG15 ubiquitin-like modifier Kit CLIA, ISG15 Kit CLIA, isg15 Kit CLIA, Isg15 Kit CLIA

Sujet:

Gene Name: Ubiquitin Cross Reactive Protein

Gene Aliases: G1P2, IFI15, ISG15, Interferon Alpha-Inducible Protein clone IFI-15K, Interferon Induced Protein 15, Interferon-induced 17 kDa protein

ID gène: 9636

UniProt: P05161