ube3a Kit ELISA

N° du produit ABIN5659743

Détail du produit ube3a Kit ELISA

Antigène: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ubiquitin Protein Ligase E3A (UBE3A). No significant cross-reactivity or interference between Ubiquitin Protein Ligase E3A (UBE3A) and analogues was observed.

Sensibilité: 0.114 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Protein Ligase E3A (UBE3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Protein Ligase E3A (UBE3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Protein Ligase E3A (UBE3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Protein Ligase E3A (UBE3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Protein Ligase E3A (UBE3A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Protein Ligase E3A (UBE3A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Autre désignation: Ubiquitin Protein Ligase E3A (ube3a Produits)

Synonymes: ANCR Kit ELISA, AS Kit ELISA, E6-AP Kit ELISA, EPVE6AP Kit ELISA, HPVE6A Kit ELISA, DDBDRAFT_0188760 Kit ELISA, DDBDRAFT_0302447 Kit ELISA, DDB_0188760 Kit ELISA, DDB_0302447 Kit ELISA, ube3a Kit ELISA, im:7140733 Kit ELISA, zgc:92173 Kit ELISA, 4732496B02 Kit ELISA, 5830462N02Rik Kit ELISA, A130086L21Rik Kit ELISA, Hpve6a Kit ELISA, mKIAA4216 Kit ELISA, As Kit ELISA, CG6190 Kit ELISA, Dmel\\CG6190 Kit ELISA, Dube3a Kit ELISA, UBE3A Kit ELISA, dUBE3A Kit ELISA, das Kit ELISA, dube3A Kit ELISA, dube3a Kit ELISA, MGC69536 Kit ELISA, UME3A Kit ELISA, ubiquitin protein ligase E3A Kit ELISA, ubiquitin-protein ligase E3A Kit ELISA, Ubiquitin protein ligase E3A Kit ELISA, ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) S homeolog Kit ELISA, ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) Kit ELISA, UBE3A Kit ELISA, ube3a Kit ELISA, LOC100194730 Kit ELISA, Ube3a Kit ELISA, ube3a.S Kit ELISA, Eint_040430 Kit ELISA

Sujet:

Gene Name: Ubiquitin Protein Ligase E3A

Gene Aliases: ANCR, AS, E6-AP, EPVE6AP, HPVE6A, Papilloma Virus E6-Associated,Angelman Syndrome, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54

ID gène: 7337

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway