UGGT1 Kit ELISA

N° du produit ABIN5659754

Détail du produit UGGT1 Kit ELISA

Antigène: UGGT1 (UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1). No significant cross-reactivity or interference between UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1) and analogues was observed.

Sensibilité: 0.113 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: UGGT1 (UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1))

Autre désignation: UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1 Produits)

Synonymes: UGCGL1 Kit ELISA, ugcgl1 Kit ELISA, uggt2 Kit ELISA, 0910001L17Rik Kit ELISA, A930007H10Rik Kit ELISA, AA589501 Kit ELISA, AI414429 Kit ELISA, AI448372 Kit ELISA, C820010P03Rik Kit ELISA, GT Kit ELISA, UGT1 Kit ELISA, Ugcgl1 Kit ELISA, Uggt Kit ELISA, rUGT1 Kit ELISA, HUGT1 Kit ELISA, EMS-mutagenized bri1 suppressor 1 Kit ELISA, F3I17.13 Kit ELISA, F3I17_13 Kit ELISA, PRIORITY IN SWEET LIFE 2 Kit ELISA, PSL2 Kit ELISA, UDP-GLUCOSE:GLYCOPROTEIN GLUCOSYLTRANSFERASE Kit ELISA, UGGT Kit ELISA, UDP-glucose glycoprotein glucosyltransferase 1 Kit ELISA, UDP-glucose glycoprotein glucosyltransferase 1 L homeolog Kit ELISA, UDP-glucose:glycoprotein glucosyltransferase 1 Kit ELISA, EMS-MUTAGENIZED BRI1 SUPPRESSOR 1 Kit ELISA, UGGT1 Kit ELISA, uggt1.L Kit ELISA, uggt1 Kit ELISA, LOC100545947 Kit ELISA, Uggt1 Kit ELISA, EBS1 Kit ELISA

Sujet:

Gene Name: UDP-Glucose Glycoprotein Glucosyltransferase 1

Gene Aliases: UGTR, HUGT1, GT, UGCGL1, UGGT, UGT1, UDP-glucose ceramide glucosyltransferase-like 1

ID gène: 56886

UniProt: Q9NYU2

Pathways: Activation of Innate immune Response