UROD Kit ELISA

N° du produit ABIN5659792

Détail du produit UROD Kit ELISA

Antigène: UROD (Uroporphyrinogen Decarboxylase (UROD))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Uroporphyrinogen Decarboxylase (UROD). No significant cross-reactivity or interference between Uroporphyrinogen Decarboxylase (UROD) and analogues was observed.

Sensibilité: 0.117 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uroporphyrinogen Decarboxylase (UROD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uroporphyrinogen Decarboxylase (UROD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uroporphyrinogen Decarboxylase (UROD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Uroporphyrinogen Decarboxylase (UROD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uroporphyrinogen Decarboxylase (UROD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uroporphyrinogen Decarboxylase (UROD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: UROD (Uroporphyrinogen Decarboxylase (UROD))

Autre désignation: Uroporphyrinogen Decarboxylase (UROD Produits)

Synonymes: UROD Kit ELISA, pct Kit ELISA, wu:fc43e09 Kit ELISA, PCT Kit ELISA, UPD Kit ELISA, AI323803 Kit ELISA, Uro-d Kit ELISA, porphyrinogen carboxy-lyase Kit ELISA, uroporphyrinogen decarboxylase Kit ELISA, Uroporphyrinogen decarboxylase UroD Kit ELISA, Uroporphyrinogen decarboxylase Kit ELISA, UROD Kit ELISA, urod Kit ELISA, hemE Kit ELISA, uroD Kit ELISA, Cpin_6502 Kit ELISA, Rmar_1187 Kit ELISA, LOC5568261 Kit ELISA, Mrub_1403 Kit ELISA, Arnit_2230 Kit ELISA, Ndas_2830 Kit ELISA, Mesil_2989 Kit ELISA, Trad_0222 Kit ELISA, Weevi_0432 Kit ELISA, Hipma_1229 Kit ELISA, Fluta_0529 Kit ELISA, Marky_1143 Kit ELISA, Halhy_5610 Kit ELISA, Mesop_0899 Kit ELISA, Ccan_20670 Kit ELISA, Urod Kit ELISA

Sujet:

Gene Name: Uroporphyrinogen Decarboxylase

Gene Aliases: PCT, Uroporphyrinogen III Decarboxylase

UniProt: P32362