ETS1 Kit ELISA

N° du produit ABIN5659804

Détail du produit ETS1 Kit ELISA

Antigène: ETS1 (V-Ets erythroblastosis Virus E26 Oncogene Homolog 1 (Avian) (ETS1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1). No significant cross-reactivity or interference between V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) and analogues was observed.

Sensibilité: 0.052 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ETS1 (V-Ets erythroblastosis Virus E26 Oncogene Homolog 1 (Avian) (ETS1))

Autre désignation: V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1 Produits)

Synonymes: ETS-1 Kit ELISA, EWSR2 Kit ELISA, ets1a-a Kit ELISA, XE1-a Kit ELISA, X1-c-ets-1a Kit ELISA, ETS-1A Kit ELISA, ETS-1B Kit ELISA, TNIP1 Kit ELISA, c-ets-1 Kit ELISA, c-ets1 Kit ELISA, Ets-1 Kit ELISA, Etsoncb Kit ELISA, Tpl1 Kit ELISA, ets1 Kit ELISA, ets Kit ELISA, AI196000 Kit ELISA, AI448617 Kit ELISA, D230050P06 Kit ELISA, cb516 Kit ELISA, id:ibd1116 Kit ELISA, zgc:110573 Kit ELISA, XE1-b Kit ELISA, ets-1 Kit ELISA, ets1-B Kit ELISA, ETS proto-oncogene 1, transcription factor Kit ELISA, v-ets avian erythroblastosis virus E26 oncogene homolog 1 S homeolog Kit ELISA, v-ets avian erythroblastosis virus E26 oncogene homolog 1 Kit ELISA, v-ets erythroblastosis virus E26 oncogene homolog 1 (avian) Kit ELISA, E26 avian leukemia oncogene 1, 5' domain Kit ELISA, v-ets avian erythroblastosis virus E26 oncogene homolog 1 L homeolog Kit ELISA, ETS1 Kit ELISA, ets1.S Kit ELISA, Ets1 Kit ELISA, ets1 Kit ELISA, ets1.L Kit ELISA

Sujet:

Gene Name: V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1

Gene Aliases: EWSR2, p54, Protein C-ets-1

ID gène: 23871

UniProt: P27577