K-RAS Kit ELISA

N° du produit ABIN5659809

Détail du produit K-RAS Kit ELISA

Antigène: K-RAS (KRAS) (GTPase Kras (KRAS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS). No significant cross-reactivity or interference between V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) and analogues was observed.

Sensibilité: 0.125 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: K-RAS (KRAS) (GTPase Kras (KRAS))

Autre désignation: V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS Produits)

Synonymes: wu:fa04e08 Kit ELISA, wu:fc14b12 Kit ELISA, wu:fc23g10 Kit ELISA, wu:fj89d12 Kit ELISA, zgc:85725 Kit ELISA, Kras2 Kit ELISA, c-Ki-ras Kit ELISA, p21 Kit ELISA, C-K-RAS Kit ELISA, CFC2 Kit ELISA, K-RAS2A Kit ELISA, K-RAS2B Kit ELISA, K-RAS4A Kit ELISA, K-RAS4B Kit ELISA, KI-RAS Kit ELISA, KRAS1 Kit ELISA, KRAS2 Kit ELISA, NS Kit ELISA, NS3 Kit ELISA, RASK2 Kit ELISA, AI929937 Kit ELISA, K-ras Kit ELISA, Ki-ras Kit ELISA, Kras-2 Kit ELISA, p21B Kit ELISA, ras Kit ELISA, k-ras Kit ELISA, kras Kit ELISA, kras-a Kit ELISA, kras-b Kit ELISA, rask2 Kit ELISA, K-RAS Kit ELISA, p21ras Kit ELISA, KRAS proto-oncogene, GTPase Kit ELISA, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog Kit ELISA, Kirsten rat sarcoma viral oncogene homolog Kit ELISA, semicolonial-7 Kit ELISA, Ras family protein Kit ELISA, GTPase KRas Kit ELISA, Kirsten rat sarcoma viral oncogene homolog S homeolog Kit ELISA, KRAS Kit ELISA, kras Kit ELISA, Kras Kit ELISA, smco-7 Kit ELISA, PGTG_03066 Kit ELISA, Tsp_02666 Kit ELISA, Tsp_01642 Kit ELISA, rask Kit ELISA, kras.S Kit ELISA

Classe de substances: Viral Protein

Sujet:

Gene Name: V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog

Gene Aliases: C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2

ID gène: 3845

UniProt: P01116