VCP Kit ELISA

N° du produit ABIN5659824

Détail du produit VCP Kit ELISA

Antigène: VCP (Valosin Containing Protein (VCP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Valosin Containing Protein (VCP). No significant cross-reactivity or interference between Valosin Containing Protein (VCP) and analogues was observed.

Sensibilité: 0.112 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Valosin Containing Protein (VCP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Valosin Containing Protein (VCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Valosin Containing Protein (VCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Valosin Containing Protein (VCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Valosin Containing Protein (VCP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Valosin Containing Protein (VCP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: VCP (Valosin Containing Protein (VCP))

Autre désignation: Valosin Containing Protein (VCP Produits)

Synonymes: ALS14 Kit ELISA, IBMPFD Kit ELISA, TERA Kit ELISA, p97 Kit ELISA, CDC48 Kit ELISA, wu:fd16d05 Kit ELISA, wu:fj63d11 Kit ELISA, p93 Kit ELISA, 3110001E05 Kit ELISA, p97/VCP Kit ELISA, 22.26 Kit ELISA, 7.12 Kit ELISA, Apple Kit ELISA, BcDNA.GM02885 Kit ELISA, BcDNA:GM02885 Kit ELISA, CG2331 Kit ELISA, DmTER94 Kit ELISA, Dmel\CG2331 Kit ELISA, I Kit ELISA, Ter94 Kit ELISA, Tera Kit ELISA, VCP Kit ELISA, VCP ATPase Kit ELISA, VCP/p97 Kit ELISA, Vcp Kit ELISA, anon-WO2004063362.65 Kit ELISA, anon-WO2004063362.67 Kit ELISA, cdc48 Kit ELISA, dTERA Kit ELISA, dVCP Kit ELISA, dmTERA Kit ELISA, dvcp Kit ELISA, l(2)03775 Kit ELISA, l(2)46CFf Kit ELISA, l(2)46CFs Kit ELISA, l(2)46Ch Kit ELISA, pTER94 Kit ELISA, ter94 Kit ELISA, vcp Kit ELISA, FANCG Kit ELISA, valosin containing protein Kit ELISA, valosin containing protein S homeolog Kit ELISA, valosin-containing protein Kit ELISA, CG2331 gene product from transcript CG2331-RA Kit ELISA, zgc:136908 Kit ELISA, transitional endoplasmic reticulum ATPase Kit ELISA, VCP Kit ELISA, vcp.S Kit ELISA, vcp Kit ELISA, Vcp Kit ELISA, Bm1_56585 Kit ELISA, LOC100136246 Kit ELISA, TER94 Kit ELISA, zgc:136908 Kit ELISA, LOC101109680 Kit ELISA

Sujet:

Gene Name: Valosin Containing Protein

Gene Aliases: IBMPFD, p97, TERA, Transitional Endoplasmic Reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit

ID gène: 7415

UniProt: P55072

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway