FLT1 Kit CLIA

N° du produit ABIN5659879

Détail du produit FLT1 Kit CLIA

Antigène: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12 pg/mL - 20000 pg/mL

Seuil minimal de détection: 78.12 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Follicular Fluid, Plasma, Saliva, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor Receptor 1 (VEGFR1). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) and analogues was observed.

Sensibilité: 10.5 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor Receptor 1 (VEGFR1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor Receptor 1 (VEGFR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Autre désignation: Vascular Endothelial Growth Factor Receptor 1 (FLT1 Produits)

Synonymes: FLT Kit CLIA, FLT-1 Kit CLIA, VEGFR-1 Kit CLIA, VEGFR1 Kit CLIA, FLT1 Kit CLIA, flt1b Kit CLIA, sFlt1 Kit CLIA, AI323757 Kit CLIA, Flt-1 Kit CLIA, fms related tyrosine kinase 1 Kit CLIA, FMS-related tyrosine kinase 1 Kit CLIA, vascular endothelial growth factor receptor 1 Kit CLIA, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) Kit CLIA, FMS-like tyrosine kinase 1 Kit CLIA, vascular endothelial growth factor receptor-1 Kit CLIA, FLT1 Kit CLIA, Flt1 Kit CLIA, CpipJ_CPIJ012087 Kit CLIA, flt1 Kit CLIA, FLT-1 Kit CLIA

Sujet:

Gene Name: Vascular Endothelial Growth Factor Receptor 1

Gene Aliases: Flt1, Fms-Related Tyrosine Kinase 1, Vascular Permeability Factor Receptor, Fms-like tyrosine kinase 1, Tyrosine-protein kinase receptor FLT

Pathways: Signalisation RTK, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals