VASP Kit ELISA

N° du produit ABIN5659905

Détail du produit VASP Kit ELISA

Antigène: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Vasodilator Stimulated Phosphoprotein (VASP). No significant cross-reactivity or interference between Vasodilator Stimulated Phosphoprotein (VASP) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vasodilator Stimulated Phosphoprotein (VASP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vasodilator Stimulated Phosphoprotein (VASP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vasodilator Stimulated Phosphoprotein (VASP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vasodilator Stimulated Phosphoprotein (VASP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vasodilator Stimulated Phosphoprotein (VASP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vasodilator Stimulated Phosphoprotein (VASP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Autre désignation: Vasodilator Stimulated Phosphoprotein (VASP Produits)

Synonymes: vasp Kit ELISA, MGC80889 Kit ELISA, wu:fb23b04 Kit ELISA, wu:fk84g05 Kit ELISA, zgc:110347 Kit ELISA, si:dkey-113g17.1 Kit ELISA, si:ch211-202c21.1 Kit ELISA, DDBDRAFT_0188463 Kit ELISA, DDBDRAFT_0229340 Kit ELISA, DDB_0188463 Kit ELISA, DDB_0229340 Kit ELISA, VASP Kit ELISA, vasodilator stimulated phosphoprotein S homeolog Kit ELISA, vasodilator stimulated phosphoprotein b Kit ELISA, protein enabled Kit ELISA, vasodilator-stimulated phosphoprotein Kit ELISA, vasodilator stimulated phosphoprotein a Kit ELISA, vasodilator stimulated phosphoprotein Kit ELISA, vasp.S Kit ELISA, vaspb Kit ELISA, LOC5573842 Kit ELISA, CpipJ_CPIJ004706 Kit ELISA, CpipJ_CPIJ004707 Kit ELISA, vasP Kit ELISA, vasp Kit ELISA, VASP Kit ELISA, vaspa Kit ELISA, Vasp Kit ELISA

Sujet:

Gene Name: Vasodilator Stimulated Phosphoprotein

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation