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WT1 Kit CLIA

WT1 Reactivité: Humain Chemiluminescent Sandwich ELISA 156.25 pg/mL - 10000 pg/mL Tissue Homogenate
N° du produit ABIN5659991
  • Antigène Voir toutes WT1 Kits CLIA
    WT1 (Wilms Tumor 1 (WT1))
    Reactivité
    • 5
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Chemiluminescent
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156.25 pg/mL - 10000 pg/mL
    Seuil minimal de détection
    156.25 pg/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Wilms Tumor Protein (WT1). No significant cross-reactivity or interference between Wilms Tumor Protein (WT1) and analogues was observed.
    Sensibilité
    6.21 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    2 - 3 h
    Plaque
    Pre-coated
    Protocole
    The microplate provided in this kit has been pre-coated with an antibody specific to Wilms Tumor Protein (WT1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wilms Tumor Protein (WT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wilms Tumor Protein (WT1) level in the sample or standard.,
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes WT1 Kits CLIA
    WT1 (Wilms Tumor 1 (WT1))
    Autre désignation
    Wilms Tumor Protein (WT1 Produits)
    Sujet

    Gene Name: Wilms Tumor Protein

    Gene Aliases: WAGR, GUD, WIT-2, WT33

    ID gène
    7490
    UniProt
    P19544
    Pathways
    Tube Formation
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